Lysyl endopeptidase is an enzyme that hydrolyzes lysyl peptide bonds of nonterminal amino acids. It is highly specific and preferentially cleaves Lys-I-Xaa bonds, including Lys-I-Pro. Used in research as a standard laboratory reagent for enzyme digestion. This is useful to study the primary stru...
18 hours at 37 °C depending on the enzyme/ substrate ratio. Notes: Self-digestion of may occur if temperatures >37 °C are used.Lys-C retains most of its activity in 2.0 M urea or 0.1% SDS.2,3,6 A peptide such as melittin or the oxidized B chain of insulin should be run ...
这里的一系列酶指的是泛素化级联反应过程中相互协同的3种酶:E1泛素激活酶(Ubiquitin-activating enzyme)、E2泛素偶联酶 (Ubiquitin-conjugating enzymes)和E3泛素连接酶(Ubiquitin-ligase enzymes)。 泛素连接酶E3主要有两大类:HECT结构域家族和RING结构域家族,最近又发现了一类新的E3家族:U.box蛋白家族。HECT结构域主...
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea...
例如,带有His、GST、HA、Flag、Myc、V5等标签蛋白的分离纯化,免疫印迹(Western blot)、免疫沉淀(Immunoprecipitation)、免疫共沉淀(Co-immunoprecipitation)和ELISA (Enzyme linked immunosorbent assay)等常规生化分析,luciferase、β-gal、alkaline phosphatase、chloramphenicol acetyltransferase (CAT)等报告基因(Report gene)...
Arg-C specifically cleaves at the carboxyl side of Arginine residues. Arg-C has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl-containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydry...
Arg-C specifically cleaves at the carboxyl side of Arginine residues. Arg-C has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl-containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl react...
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea...
The enzyme cleaves b (1,4) linkage between N-acetylglucosamine and N-acetylmuramic acid of the peptidoglycan component in the bacterial cell wall. It exhibits four times more activity than commercial chicken lysozyme and is useful in the recovery of proteins expressed in bacteria (e.g. E. ...
EZLys Lysozyme is a recombinant human lysozyme expressed and purified in an animal-free system (rice). It has an identical amino acid sequence and physic-chemical properties of native lysozyme. The enzyme cleaves b (1,4) linkage between N-acetylglucosamine and N-acetylmuramic acid of the pepti...