胰蛋白酶(trypsin)是目前“Bottom-up”策略中使用最广泛的蛋白水解酶,能特异性酶切赖氨酸和精氨酸C端的肽键(Lys-X和Arg-X)。它的两个主要优势是:(1)酶切位点特异性高;(2)酶解得到的肽段产物两端各有一个碱性基团,有利于质谱的鉴定。但是由于胰酶在赖氨酸上的酶切效率比较低,因此常常产生不完全酶解的肽段,...
The automated Iin situ endoproteinase Lys-C digestion of glutamine synthetaseHewlett, Packard
Matched pairs of fragmentation spectra with equal precursor mass and similar retention times from each digestion are compared, leveraging single-residue transposed information with independent interferences to confidently identify fragment ion type, residues, and peptides. The paired spectra are solved ...
Lysyl endopeptidase is an enzyme that hydrolyzes lysyl peptide bonds of nonterminal amino acids. It is highly specific and preferentially cleaves Lys-I-Xaa bonds, including Lys-I-Pro. Used in research as a standard laboratory reagent for enzyme digestion. This is useful to study the primary stru...
18 hours at 37 °C depending on the enzyme/ substrate ratio. Notes: Self-digestion of may occur if temperatures >37 °C are used.Lys-C retains most of its activity in 2.0 M urea or 0.1% SDS.2,3,6 A peptide such as melittin or the oxidized B chain of insulin should be run ...
The rTrx was removed by digestion with enterokinase, and mature Lys1 (Lys1, ~ 14 kDa) was obtained. The growth of L. plantarum was significantly suppressed after incubation with Lys1 (Fig. 4E, F). These results revealed the contri- bution of CD209-controlled Lys1 in restricting ...
Mithochondiral mutation was determined by RFLP of PCR DNA product with mismatched primer following Nae I restriction enzyme digestion of Genomic DNA from PB. The mutation load (Ratio of mutant DNA/nl DNA) was found to be variable in the family. 60% in the proband, 80% in mildly affected...
Met—Lys一双C肽人胰岛素原 基因的构建表达及分离纯化 陈雅蕙杜雅励.唐建国 ,积±jEl0o871) )} 摘要应用PCR定点突变方法构建编码Met—Lys一双C肚人胰岛素原基因,并在 大肠杆菌中以包 含体方式获得表达.表达产物经还原,重组,SephadexG一75分离纯化,获得Met— ...
19 the human Ig k- chain C region (Ck), a sequence for another linker (Gly-Gly-Gly-Ser)3 and the restriction sites for AgeI (NEB), NotI (NEB) and BamHI were chemically synthesized (Genscript, Piscataway, NJ, USA) and cloned into the pCEP4 vector after digestion with the ...
Fragments of lys-plasminogen refers to portions of the amino acid sequence of the lys-plasminogen polypeptide. These fragments can be generated directly from lys-plasminogen itself by chemical cleavage, by proteolytic enzyme digestion, or by combinations thereof. Additionally, such fragments can be crea...