Lymphorgans(thymus,lymphnode,andspleen) Function: Immunologicdefence Immunesurveillance Immunologichomeostasis Introduction: Theclassificationoflymphatictissue Thehistologyofthelymphaticorgan: thethymus thelymphnode thespleen Theclassificationofimmunecells Tcells,and ...
the lymphoid organs were removed. They were stored at −80 ◦ C (Sanyo, Japan) in cryovials for further analysis. 2.4. RNA extraction and cDNA synthesis Total RNA was extracted from lymphoid organ using Trizol reagent (Invitrogen, USA) as per manufacturer’s instructions. Com- plementary...
LTi cells induce adhesion molecules, chemokines, and the recruitment of other cells to establish the secondary lymphoid organs, via lymphotoxin signaling to mesenchymal organizer cells.10 Like Th17 cells, which also express Rorgt, fetal LTi cells produce IL-17A and IL-22.11,12 Cells with an LTi...
Immunoreagents The following mouse anti-pig monoclonal Abs, whose source and specificity were described earlier [34],[35], were used as primary immunoreagents: anti-CD2 (1038H-5-37, IgM), anti-CD3 (PPT3, IgG1), anti-CD4 (10.2H2, IgG2b), anti-CD8α (76-2-11, IgG2a), anti-CD21 (...