The blood lymphocytes under the light- and electron microscope after stimulation with phytohemagglutinindoi:10.1007/BF02044579On the basis of light and electron microscopical observations, a difference between the blood lymphocytes of normal individuals and patients with Hodgkin's disease and chronic ...
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Stained sections were mounted and observed under a light microscope. For detecting the type of lymphocytes that was proliferating in the inflamed pituitaries, sections were first incu- bated with rat anti-CD3 (AbD Serotec) or rat anti-B220 (1:100, eBioscience, San Diego, CA, U. S. A.)...
Images were taken at the Axio Observer.Z1 fluorescence microscope with ApoTome technology or LSM 780 confocal laser scanning microscope (both from Carl Zeiss). Images were quantified using the cell counter plugin and the ImageJ software (version 5.2.1). Cell death analysis To determine the cell...
Tumor tissue sections stained with hematoxylin and eosin (H&E) were independently evaluated by two pathologists under light microscope to score TILs’ percent. TILs ≥ 10% was adopted as invasive breast cancer with high TILs, except in TNBC where the bar was adjusted to ≥ 17.5% to...
Journal of Ovarian Research (2020) 13:65 Page 4 of 11 Histological evaluation for tumor-infiltrating lymphocytes Complete sections of H&E-stained slides were reviewed under a light microscope. A representative tumor sec- tion was selected to assess the stromal TIL percentage, which, as recommended...
There was also a difference of 0.3 Gy between samples with and without CBCT. Comparing the summarized therapeutic doses from the TPS and imaging doses written out by LINAC console, we found that they did not correlate with the measured doses. As the frequency of the images increased in ...
Following FISH procedure, the cells were examined for the volumes of two territories in the nuclei using the optical section images on a confocal laser scanning microscope (CLSM). And we analyzed the variation in the volume ratio (R) of two territories at different stages in . Our data ...
Trypan blue (TB) is a commonly used dye to distinguish between live and dead cells. One of the most common methods of counting cells using TB is manual counting using hemacytometer slide. As this method involves counting of cells by a researcher under a microscope, this is a time-consuming...
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the...