However, in our study, there were no significant changes in the counts of neutrophils, monocytes, and platelets, whereas lymphocyte counts increased in the IMH group. We assumed that during the MH formation phase, lymphocyte activation increased the release of related inflammatory factors and ...
Recombinant human interferon-inducible protein 10 is a chemoattractant for human monocytes and T lymphocytes and promotes T cell adhesion to endothelial ce... The human cytokine interferon-inducible protein 10 (IP-10) is a small glycoprotein secreted by activated T cells, monocytes, endothelial cells...
The PBMC fraction, including lymphocytes and monocytes, was separated by standard Ficoll-Hypaque gradient centrifugation following the manufacturer’s protocol (Amersham Pharmacia, Uppsala, Sweden). Briefly, 5 ml of Ficoll-Hypaque gradient was prepared in 15-ml centrifuge tubes. EDTA blood was diluted ...
(C) Fifty GBM patients were classified into two major groups (red and black clusters) by unsupervised hierarchical clustering according to the expression pattern of the Constant_imm_genes. The expression levels of these genes are illustrated as a color spectrum, with red, white and blue ...
TIM3, the member of the TIM family of immunomodulatory proteins encoded by HAVCR2, is mainly expressed in CD8+T lymphocytes, CD4+T lymphocytes, NK cells, and monocytes [16]. In many tumors such as melanoma [17] and gastric cancer [18], the increase of TIM3 is associated with poor prog...
IFN-γ is the most potent stimulator for monocytes/macrophages. Excessive release of this cytokine will undoubtedly lead to monocyte/macrophage activation with concomitant production of pro-inflammatory mediators, including IL-6. Such an acute phase response in UA patients could be regarded as a ...
Protein bound polysaccharides (PBP), among them PSP (polysaccharide peptide) and PSK (Krestin) derived from Coriolus versicolor (CV) fungus are classified as biological response modifiers (BRMs), with potential therapeutic applications [1], [2]. Both, PSP and PSK, are proteoglycans of approximatel...
[18]. CRAC motif mediates LtxA binding to cholesterol and is essential for LtxA association with the plasma membrane of human T lymphocytes and monocytes [18,19]. The central domain (residues 421–730) contains two internal lysine residues (K562and K687) that are the sites of post-...
Intracellular IFN-γ staining and fluorescence-activated cell sorter analysis The Cytofix/Cytoperm kit (PharMingen, San Diego, CA) was used for intracellular IFN-γ staining. Thawed cells were incubated for 3 h in a 24 well culture plate at 37°C. This leads to adherence of monocytes. The...
1b, c), we classified immune cells into four categories: T-cells (T-cell, 62,489 cells), B cells (B-cell, 14,097 cells), dendritic cells (DC, 6637 cells), and monocytes/macrophages (mono/macro, 26,836 cells) (Figs. 1c, S1d). The annotated major cell types were validated ...