The nuclei and membranes of epd-positive cells are labeled with H2B-GFP and mCherry-Ras, respectively. Enlarged area showing irregular spindle-shaped epd-positive single-cell. n = 15 per stage. c Schematic diagram showing the strategy for ablation of epd-positive cells. d, e ...
Finally, we discovered that in advanced breast cancer, the proportion of pCAF increased and was associated with a poor prognosis of breast cancer. This study elucidated the potential cellular origins and drivers of breast cancer metastases to lymphatic nodes, brain, and bone, utilizing single-cell ...
Schematic diagram summarizing the results that indicate that tumor cells undergoing TGF-β1-induced EMT become activated for targeted migration through the lymphatic system, similar to DCs during inflammation. Induction of CCR7 provides both EMT cells and DCs with a capacity to sense and migrate towar...
New valves in the zebrafish lymphatic system.aStructure of the remaining facial lymphatic sprout (RFLS, arrowheads) during zebrafish larvae development as labeled byTg(lyve1b:TopazYFP). The white and yellow arrows indicate the sprouts or connections of the RFLS with the otolithic lymphatic vessel ...
CA) and then incubated with horseradish peroxidase-conjugated anti-goat secondary antibodies (1:2000; Santa Cruz Biotechnology). Blots were visualized with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, MA) and analyzed with the ChemiDoc System (Bio-Rad, Hercules, CA)...