In yeast two-hybrid assays, core LXXLL motif sequences derived from steroid receptor co-activator (SRC1), the 140-kDa receptor interacting protein (RIP140), and CREB-binding protein (CBP) displayed differences in selectivity and affinity for nuclear receptor ligand binding domains. Although core ...
Several recently reported 3D structures of protein–LxxLL motif complexes and an intriguing novel interaction implicated in leukaemia have further highlighted the diversity and regulatory importance of this seemingly simple motif.关键词: Animals Humans Transcription Factors Repressor Proteins Trans-Activators ...
Ko L, Cardona GR, Chin WW (2000) Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator. Proc Natl Acad Sci U S A 97: 6212-6217.Ko L, Cardona GR, Chin WW. Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein...
Evidence suggested that the sequences flanking the LXXLL motif in a number of coactivators determine receptor selectivity. We performed mutagenesis studies at residues adjacent to the TRBP LXXLL motif and identified S884 of TRBP at the -3 position of the LXXLL motif as a key residue for receptor...
However, a close inspection of the electron density map indicates that the bound LXXLL motif is not a selected one but an averaged structure of more than one LXXLL motif. Further biochemical and functional studies show that the individual LXXLL motifs can bind but drive only minimal ...
The signature motif, LLL, which is responsible for binding of several coactivators to nuclear receptors, is not required for RIP140 binding to AhR. These results demonstrate that the AhR recruits coactivators that are capable of enhancing transcription and, thus, the AhR may compete with steroid ...
Although usually an antagonist, hydroxyflutamide was more potent in the recruitment of D11FxxLF or an SRC3-1 LXXLL motif to AR T877A LBD than AR LBD. The antagonist cyproterone acetate behaved as a full antagonist of D11FxxLF recruitment to AR LBD and AR T877A LBD but as a more ...
Additional file 6: Figure S6: The LXXLL motif of Stat6 is required for Stat6 to transcriptionally modulate p21 and p27. A, The LXXLL motif in Stat6 is mutated as indicated. B, Transcriptional activity analysis of wild-type or mutated Stat6 fusion protein in T47D cells. Each construct co...
2004. The coactivator LXXLL nuclear re- ceptor recognition motif. J. Pept. Res. 63: 207-212.Savkur RS,Burris TP.The coactivator LXXLL nuclear receptor recognition motif. Journal of Peptide Research . 2004Savkur RS,Burris TP. The coactivator LXXLL nuclear receptor recognition motif[J]. J Pept...
(2014) Integrity of the LXXLL motif in Stat6 is required for the inhibition of breast cancer cell growth and enhancement of differentiation in the context of progesterone. BMC Cancer 14 , 10 /Min Wei , Qi He , Zhongyin Yang , Zhiwei Wang , Qing Zhang , Bingya Liu , Qinlong Gu , ...