因此,在HF中逆转HSCs的活化,抑制HSCs增殖,促进已活化的HSCs凋亡,减轻或者逆转肝纤维化而达到逆转肝硬化或者阻止肝硬化进一步恶化的目的是非常有研究价值.天然植物提取物具有低毒性,易得等特点一直作为临床药物研发的研究热点,针对大蒜提取物蒜氨酸的研究有很多,其保肝作用,抗肿瘤作用等引起我们的注意.本实验以正常人肝...
In primary isolated HSCs, NDV also had increased replication with HSC passage and TGF-pi stimulation. Conclusion NDV replicate in activated HSC effectively, suggests that activation of HSC facilitate the replication of NDV in this kind of cells. 【期刊名称】《基础医学与临床》 【年(卷),期】2012...
本实验中我们用LPS刺激人肝星状细胞LX-2, 结果发现, 在一定时间内, 用适当浓度的LPS刺激HSCs相应时间后, 可引起HSCs的增殖活化明显增强. 但随着LPS刺激浓度增加以及刺激时间的延长, LPS刺激HSCs后同样会引起HSCs的增殖活化下降. 实验中我们进一步应用Western blot检测了凋亡指标active Caspase3, 结果显示用1.0 μg/...
HSCs become "activated" in response to tissue damage or pathogen invasion, a process most commonly driven by transforming growth factor-β1 (TGF-β1). Despite this, the full extent of TGF-β1 signalling in these cells is poorly understood. Clarifying the range and diversity of this signalling...
肝纤维化的发病机制非常复杂,目前主要认为肝星形细胞(hepatic stellate cells,HSCs)的激活,转化为成肌纤维细胞和成纤维细胞[5-8],合成分泌大量ECM在LF的发病过程中起了主要作用。肝纤维化是一种可逆的损伤修复反应[9]。HSC的激活是本病发展的中心环节,因此,主要在以下几个方面进行肝纤维化的治疗:①去除原发病因;...
Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the sta... XY Yang,Z Chen,J Tan,... - 《Current Medical Science》 被引量: 0发表: 2024年 MAF Induces Inflammatory Mediators Involved in the Patho...
The activation of hepatic stellate cells (HSCs) is the key event in the liver fibrogenesis. In this study, the behavior of human HSCs LX-2 in low oxygen tension (1% O2) was analyzed. Upon hypoxia, the expression of HIF-1α and VEGF gene was induced. The result of Western blotting ...
肝纤维化是肝脏对各种原因所致慢性损伤的修复反应,表现为以胶原蛋白为主的细胞外基质(extracellular matrix,ECM)在肝脏内过度增生和沉积,是所有慢性肝损伤向肝硬化发展的必经阶段.肝星状细胞(hepatic stellate cell,HSCs)是肝纤维化中ECM的主要来源.活化的... 覃立锋 - 广西医科大学 被引量: 0发表: 0年 CXCL12-...
我们推测miR-150对HSCs的抑制可能存在其它潜在的分子机制。 目的 本研究通过构建TGF.131诱导人肝星状细胞LX.2激活的体外模型,观测肝纤 维化相关的目的miRNA,01]miR一150的表达改变;并进一步确证其与TGF一131束1]激 是否存在浓度和时间依赖性。恢复LX一2中miR一150浓度对LX.2是否存在抑制以及 细胞外基质的表达...
In order to investigate the potential role of AGAP2 in TGFβ1-mediated fibrogenesis in HSCs, the effects of AGAP2 knock-down in LX-2 cells treated with TGFβ1 were initially studied. This cell line of human immortalised HSC has been extensively employed in the study of fibrogenesis. LX-2...