HRP-labeled secondary antibody was used at a dilution of 1:2000. Blots were developed using ProSignal® Dura ECL Reagent (Cat# 20-301B, Prometheus Protein Biology Products) and visualized using a ChemiDoc Ima
Therefore, we used our recently developed ISTAMPA methodology to discriminate between different CXCL8 proteoforms [16]. To our knowledge, we demonstrate for the first time the occurrence of site-specific proteolytic modification of CXCL8 in the BAL fluids of LTx patients with CLAD or infection, ...
developed a protocol [119] based on their findings that withdrawal of WNT signaling post lung progenitor specification at day 15 promotes a proximal fate (upregulated SOX2, P63, SCGB2A2) both in 2D and 3D culture [27]. They sorted day 15 lung progenitors, through an NKX2.1-GFP reporter ...
HRP-labeled secondary antibody was used at a dilution of 1:2000. Blots were developed using ProSignal® Dura ECL Reagent (Cat# 20-301B, Prometheus Protein Biology Products) and visualized using a ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA). Band intensity was quantified using ...
Airway challenge with a nebulised LPS solution has been used mainly to study neutrophilic inflammatory processes in the lungs and has been developed into a challenge model to investigate the effect of novel anti-inflammatory drugs under development for the treatment of diseases that are associated with...
weeks into treatment, I developed some crazy delayed allergic reaction to Bactrim leaving me with rashes, shakes, high fevers and a 2am call to my doctor. We stopped antibiotics and let my body recover until the New Year. In February at my appointment, my weight was down a little more ...
This is important because this vaccine is being developed for human use. It is encouraging to see that the MVA/S vaccine-induced antibody response contracted only minimally (less than 2-fold) between weeks 2 and 4 after boost, when maximum contraction of the antibody response happens (Kannanga...
In the generation of scaffolds, a multitude of different technologies has been developed, such as decellularization for biological scaffolds and advanced manufacturing processes such as casting, cryogelation, microfabrication, and electrospinning techniques for synthetic scaffolds (Figure 1) [12]. Upcoming...