31622 Bronchoscopy, rigid or flexible, including fluoroscopic guidance, when performed; diagnostic, with cell washing, when performed (separate procedure) 31634 Bronchoscopy, rigid or flexible, including fluoroscopic guidance, when performed; with balloon occlusion, with assessment of air leak, with admini...
In Scientific Reports | 6:23034 | DOI: 10.1038/srep23034 13 www.nature.com/scientificreports/ between washing steps were performed. The following antibodies were used α -CD11b-APC, α -CD4-FITC (eBi- oscience Inc., San Diego, USA), α -CD45-AF647, α -CD45-PerCP, α...
After which, grids were washed with 1x PBS, followed by 1% glutaraldehyde (Polysciences, Warrington, PA) incubation and washing with distilled water. For contrast stain, the grids containing the exosomes were exposed to uranyless EM stain (Electron Microscopy Sciences, Hatfield, PA), followed by...
The imaging procedure was conducted using a Zeiss Axio Imager 2 microscope (Jena, Germany) and processed with Zeiss ZEN software (Jena, Germany). Single-cell RNA sequencing Liver and lung tissues were resuscitated and washed using PBS containing 0.1% BSA, followed by cell washing with 1nmL of...
Washing steps were carried out three times for 5 min after each reagent step with washing buffer (50 mM Tris saline buffer with 0.1% (v/v) Tween 20; pH 7.6). Negative controls were included under omission of secondary antibody. Permanent AEC (perma- nent AEC Kit, Zytomed Systems) was ...
Washing fragile tumor tissue pieces vigorously can lead to the detachment of small tissue fragments in the solution, resulting in the loss of material. Mechanical dissociation of normal lung and tumor tissue samples Timing: 15 min Mechanical digestion of the sample will break down the larger tissue...
The imaging procedure was conducted using a Zeiss Axio Imager 2 microscope (Jena, Germany) and processed with Zeiss ZEN software (Jena, Germany). Single-cell RNA sequencing Liver and lung tissues were resuscitated and washed using PBS containing 0.1% BSA, followed by cell washing with 1nmL of...
by washing-in the inert gas into the ventilatory circuit. The He tracer gas flow was interrupted, and the washout of the tracer gas was recorded. EELVMBW,He was calculated from the changes in the He concentration during the wash-out phase using the software, (Spiroware, V1.4.3, ECO ...
After washing with PBS, the cells were resuspended in homogenization buffer (10 mM Tris-HCl (pH 7.4), 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 mM Na-EGTA, 1 mM dithiothrei- tol, 250 mM sucrose, 0.1 mM PMSF, leupeptin (10 mg/ml), aprotinin (10 mg/ml) and trypsin inhibi- ...
Hyperventilation results in lowering of CO2 levels in the lungs due to washing away of the carbon dioxide. In a non-self-regulating patient simulator, increased lung CO2 may or may not lead to increased ventilation. If no increase occurs, the reality of the simulation is decreased, thereby ...