If the macromolecules are larger than serum albumin, the labeled molecules are retained within the microcirculation for tens of minutes after their injection into the blood. By contrast, if the tracer is not bound to macromolecules and itself has a low molecular weight, it leaks out of the ...
cells were labeled according to the cell subtypes as identified by a previous group23. For BayesPrism TAN analysis, the parameter key was set to NULL to indicate there were no malignant cells in the reference and all 21 cell types are treated equally. Final Gibbs theta values were used to ...
The black arrows indicate the fluorescently labeled A549 cells. B The number of A549 HERC5 KO cells compared to parental cells per zebrafish embryo is significantly increased in the whole body as well as separately in the brain and the body at 3 days post injection (whole body: p = ...
(D and E) (D) ChIP-seq heatmap and (E) Venn diagram for ASCL1, PROX1 and NKX2.1 peaks identified in at least 2 of 3 SCLC-A cell lines (NCI-H2107, NCI-H128, and NCI-H889) identifies unique and shared genomic binding sites. See Table S3 for peak coordinates and Table S4 for ...
S8b). To assess the recruitment of IMRCs to the lung in greater detail, GFP-labeled IMRCs were used to track the distribution of IMRCs. A number of GFP-labeled IMRCs were observed in the interstitial spaces in the lung, but not in the lung capillaries (Fig. 5d). Immunofluorescence ...
Schematic diagram of the mechanistic findings. CXCR2 showed potent chemotaxis on neutrophils. Tumor microenvironment was abundant in immunosuppressive cytokines, such as TGF-β and Arg-1, which induced polarization of neutrophils to pro-tumor N2 type and impaired anti-tumor immune response. SB225002,...
D Dio-labeled overexpressed circKIF20B PC9 cell-derived exosomes were endocytosed by the recipient PC9 cells. E The expression of circKIF20B, miR-615-3p, and MEF2A in the PC9 cells co-cultured with exosomes from the circKIF20B-OE cells, as measured by qRT-PCR. F and G EdU assay of...
FR. Hence, we designed biotin-labeled junction-specific probes and performed circRNA pull-down assay in combination with mass spectrometry (MS) to identify circZFR-interacting proteins. The most abundant protein retrieved by the circZFR probe was HNRNPLL, readily visualized by sensitive silver ...
Fluorescently labeled secondary antibodies were added and incubated for 1-2 h, with PBS cleaning to remove unbound secondary antibodies. Nuclei were stained with DAPI or other dyes. After covering with anti-fading sealant, fluorescence microscopy was used for observation and image capture to ...
The SILAC-labeled cells (heavy) and the unlabeled cells (light) were treated with phosphate-buffered saline and 100 nM DAC, respectively, for three consecutive days (D3) and grown in a drug-free medium for another three days (D3R3) (Fig. 1b). After biotinylation of the cell surface...