The muscle had been detached from the dorsum, without its insertion being resected, two weeks earlier. In the second stage, we excised a region of skin that exhibited a clear change in color together with an additional 1 cm around this area. With help from a thoracic surgeon, the adherent...
an increase in pathologic fenestrations causes the alveolar septa to appear as isolated, detached segments in two-dimensional histologic sections. Fibrosis is not a component of emphysema, but fibrosis is often present in emphysematous lungs because cigarette smoking, which is the most common cause of...
NCI-H1975 and Beas-2B cells at logarithmic growth phase were washed with PBS twice to remove serum, detached with 0.25% trypsin for 3–5 min, and added with normal culture medium to terminate detachment. Cells on the bottle wall were gently blown down, centrifuged at 100 g for 5 min ...
Then supernatants and detached cells were collected in a 50 mL falcon tube. The cells were centrifuged and the pellet was further washed with ice cold PBS before resuspending the cells at a concentration of 1 × 107 cells per mL. When applicable, cells were further incubated with ...
The edge of the gel was detached from the well walls with a pipet tip before 2 × 105 primary AT2 cells with 1 mL medium were added. After culture for 48 h, pictures of the gels were captured, and the gel area was analyzed using ImageJ software (US National Institutes of ...
To avoid confusion with artificially detached or floating cells during tumor dissection, tumor cells were considered as STAS only if they appeared as detached small clusters or if they were arranged in loose small groups within air spaces in a continuous manner from the edge of the tumor, and ...
long needle reaching into my lung. Surprisingly, I was also occasionally cognizant of pain, and even flinched once–not something you want to do when undergoing a needle biopsy. After that, I focused on holding still, and, perhaps because I was rather emotionally detached, found it easy to ...
Alveolar macrophages were also obtained from lung cell suspensions grown in 75cm2 tissue culture flask in DMEM (Invitrogen) supplemented with 10% FBS and 1% of AA, detached using trypsin (Invitrogen) and purified based on their CD45 expression by using magnetic cell separation (MACS) according ...
Although the remaining lymph nodes around the trachea were dissected and the SVC was adequately detached (Fig. 2a), confirming the adhesion between the tumor and azygos vein was difficult under direct vision from this position. After dividing the proximal site of the right main pulmonary artery ...
To avoid confusion with artificially detached or floating cells during tumor dissection, tumor cells were considered as STAS only if they appeared as detached small clusters or if they were arranged in loose small groups within air spaces in a continuous manner from the edge of the tumor, and ...