Here we describe a detailed protocol for quantifying ROS bursts induced in potato leaf discs in response to a bacterial elicitor and Phytophthora infestans.doi:10.1007/978-1-0716-2517-0_22Muhammad Awais.ZahidDepartment of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, ...
ROS burstReactive oxygen species (ROS)flg22The rapid production of reactive oxygen species (ROS) in response to biotic and abiotic cues is a conserved hallmark of plant responses. The detection and quantification of ROS generation during immune responses is an excellent readout to analyze signaling...
This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single-point measurement based on the intracellular reduction of nitroblue tetrazolium (NBT) and a real-time luminol-enhanced assay based on the detection of native ...