The nova2 gRNA was in vitro transcribed and purified from DraI-digested plasmid as template, using the Maxiscript T7 Kit (Life Technologies). Capped and polyadenylated Cas9 mRNA was in vitro tran- scribed and purified from 1 mg of PmeI-digested pMLM3613 Cas9 expression vector (Addgene, ...
Slides were mounted in prolong gold (Life Technologies). PLA was performed according to the manufacturer’s protocol (Duolink II Fluorescence, Duolink In Situ Detection Reagents Red) and PLA probes rabbit PLUS and mouse MINUS were used. Cells were fixed in 4% paraformaldehyde in PHEM buffer, ...
The steam engine was one of the most important technologies of the Industrial Revolution, inspiring other innovations and initiating further technological advancements. In 1775, James Watt formed an engine building and engineering partnership with manufacturer Matthew Boulton. This served as a kind of cr...
A few technologies are as follows: Joule–Thomson skid Cryogenic or chiller system Chemical enzymology system Power Generation Natural gas is a major source of electricity generation through the use of cogeneration, gas turbines and steam turbines. Natural gas is also well suited for a combined use...
Since the mid-1970s, the primary focus of non-proliferation efforts has been to maintain and even increase international control over the fissile material and specialized technologies necessary to build such devices, because these are the most difficult and expensive parts of a nuclear weapons program...
IBM PC compatible computers use an x86-compatible microprocessor, manufactured by Intel, AMD, VIA Technologies or Transmeta. Apple Macintosh computers were initially built with the Motorola 680×0 family of processors, then switched to the PowerPC series; in 2006, they switched to x86-compatible ...