Tsuji proposed a reaction mechanism for the production of light by the squid based on investigations into the biochemical basis of W. scintillans bioluminescence3,9, where coelenterazine disulfate is first adenylated using ATP, then reacted with oxygen to form an unstable dioxetanone Scientific ...
Reporter gene systems based upon modified luciferase genes isolated from organisms, ranging from bacteria to insects, have proven to be important tools for plant molecular studies. The biochemical characteristics of these genes combine very high sensitivity with the ability to determine reporter activity ...
Specifically, the GloMax™ 20/20 Luminometer accommodates measurement of firefly and Renilla luciferase for genetic reporter studies and ATP-based biomass analysis and so is an ideal platform for reading Promega bioluminescent assays. Scintillation Counters Ideally, the coincidence circuit of the ...
A similar circular permutation of NanoLuc was previously used to construct a protease-controlled OFF switch29. Fig. 1: Engineering of circular permutated NanoLuc and thereon based biosensors. a NanoLuc topology model29 consisting of a 10-stranded β-barrel. The dissection point used to generate ...
In general, analytical systems based on NanoLuc reporters are used in the same research areas as RLuc ones, but they successfully compete with them due to the significantly lower molecular weight and commercial availability of various variants of this enzyme provided by Promega Co along with manuals...