Tsuji proposed a reaction mechanism for the production of light by the squid based on investigations into the biochemical basis of W. scintillans bioluminescence3,9, where coelenterazine disulfate is first adenylated using ATP, then reacted with oxygen to form an unstable dioxetanone Scientific ...
Tsuji proposed a reaction mechanism for the production of light by the squid based on investigations into the biochemical basis of W. scintillans bioluminescence3,9, where coelenterazine disulfate is first adenylated using ATP, then reacted with oxygen to form an unstable dioxetanone Scientific ...
Specifically, the GloMax™ 20/20 Luminometer accommodates measurement of firefly and Renilla luciferase for genetic reporter studies and ATP-based biomass analysis and so is an ideal platform for reading Promega bioluminescent assays. Scintillation Counters Ideally, the coincidence circuit of the ...
Principles and Methods of Molecular Imaging in Stroke Luciferase Imaging-Based Cell Tracking Luciferases are oxidative enzymes used inbioluminescence.Firefly luciferaseis widely used as a bioluminescent reporter in many areas of biotechnology[1]. Firefly luciferase generates bioluminescence by catalyzing the ...
Molecular Systems Biology (2025) Inhibition of USP7 upregulates USP22 and activates its downstream cancer-related signaling pathways in human cancer cells Keqiang Zhang Ting Sun Dan J. Raz Cell Communication and Signaling (2023) Flow cytometric single cell-based assay to simultaneously detect cell...
The complexity of structural rearrangements required for alternative folding frames to switch efficiently in ligand-dependent fashion complicates the design of such biosensors, and also requires extensive optimization to achieve fast response kinetics17. The biosensors based on reg- ulatory domain insertion...
Based on this system, ponatinib and perifosine are screened out from the FDA-approved anti-cancer drug library containing 106 compounds. Concretely, they exhibit the most potent luminescent activity and cause drastic pyroptosis in PDAC cells. Further, we demonstrate that perifosine suppresses pancreatic...
rearrangements required for alternative folding frames to switch efficiently in ligand-dependent fashion complicates the design of such biosensors, and also requires extensive optimization to achieve fast response kinetics17. The biosensors based on regulatory domain insertion do not follow a general design...
Li, I.T. et al., “Protein biosensors based on the principle of fluorescene resonance energy transfer for monitoring cellular dynamics,” Biotech. Lett. (2006) 28(24):1971-1982. Littlewood, T.D. et al., “A modified oestrogen receptor ligand-binding domain as an improved switch for the...
(c) waiting for a period of time to allow the luminescent signal of the first luciferase to decline; (d) measuring the luminescent signal in the sample; and (e) calculating the luminescent signal of each luciferase based on the results of (b) and (d) and the differing rates of decay ...