Scaffold protein containing ankyrin repeats and SAM domain. A scaffold protein (461aa) found in the apical region of cochlear and vestibular hair cells but not in stereocilia. It interacts with harmonin and myosin 7A. Mutations cause a form of deafness (Usher Syndrome 1G). ...
LSm11 is a 360 amino acid protein that localizes to the nucleus and belongs to the LSm subfamily of snRNP Sm proteins. Containing an N-terminal domain that is necessary for pre-mRNA cleavage, LSm11 functions to binds specifically to U7 snRNA and is thought to play a role in cell cycle ...
Many orthopoxvirus messenger RNAs have an unusual nontemplated poly(A) tract of 5 to 40 residues at the 5′ end. The precise function of this feature is unknown. Here we show that 5′ poly(A) tracts are able to repress RNA decay by inhibiting 3′-to-5′
Many orthopoxvirus messenger RNAs have an unusual nontemplated poly(A) tract of 5 to 40 residues at the 5′ end. The precise function of this feature is unknown. Here we show that 5′ poly(A) tracts are able to repress RNA decay by inhibiting 3′-to-5′
We identified a Sm-like protein Lsm12 complexed with NAADP, TPC1, and TPC2. Lsm12 directly binds to NAADP via its Lsm domain, colocalizes with TPC2, and mediates the apparent association of NAADP to isolated TPC2 or TPC2-containing membranes. Lsm12 is essential and immediately participates...
Sm protein was first studied in research on precursor RNA. It is a highly conserved homologous protein family containing Sm motifs; therefore, it is called the Like-Smith (LSM) protein [13]. The conserved folding of Sm and LSM subunits consist of a 5-strand antiparallel β sheet, and in ...
FIGURE 1. Genomic location of the Lsm protein gene. A, the operon containing the genes for Lsm and L37e is bordered by the gene for a potential hydrolase (HVO_2724) and the gene for an amidophosphoribosyltransferase (HVO_2721). The genomic region is given below in nucleotides. B, the ...
We wanted to analyse whether the deletion of the Sm1 domain resulted in a change in expression of those sRNA, that co-purified with the Lsm protein. We isolated RNA from wild type and ΔSm1 cells and analysed these RNAs using northern blots. Probes against sRNAs 30, 34, 45 and 132 ...
Cover slips containing the cells were then washed by PBS-TX (10 mL PBS+10 μL 10% Tri- tonx-100) three times and mounted using mounting medium (PBS: Glycerol=1:1 v/v) and analyzed by fluorescence micros- copy (Leica DM IRB). Acta Pharmacologica Sinica www.chinaphar.com Li L et ...