T7EI目的:利用猿猴病毒40(Simian virus 40,SV40)Large T(LT)抗原慢病毒转染含有LoxP-Stop-LoxP(LSL)元件的Cas9转基因小鼠(LSL-Cas9)胚胎成纤维细胞(mouse embryonic fibroblast,MEF),建立永生化LSL-Cas9小鼠MEF系并用于sgRNA剪切效率的高效验证.克服由于Cas9序列过大而引起的转染效率低的问题,构建一种用于sgRNA剪切...
Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 a
Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 a
Here, we describe the development and use of a new Cre recombinase-dependent dCas9-VPR mouse model, with the potential of long-lasting transcriptional activation in vivo. This mouse model is applicable for gene induction by gRNAs to target different genes of interest, individually or in combinatio...
Mouse Targeting Cre/Lox CRISPR growth strain : Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection. ...
GSEA results for the LSL-KrasG12D; Trp53Flox/Flox geneset derived from the mouse model of soft tissue sarcoma (Table S3). Cre-lox P c KO systems, because sg RNAs-Cas9 serves as a trans approach that a single copy of sg RNAs and LSL-Cas9 can target both gene alleles... KM Jeffrey...
Transgenic animals with increased or abrogated target gene expression are powerful tools for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9-VPR mouse model for targeted induction of endogenous gene expression using different Adeno-associated virus (AAV) capsid variants f...
C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc 目录号 NM-TG-00025 品系状态 活体 导出PDF 品系描述 Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged ...