Ectopically expressed GFP-tagged Lsd1 or Lsd2 localize to lipid droplets, both in mammalian cell culture and in the fat body of Drosophila (Miura et al., 2002). This shows that the targeting to lipid droplets is a feature conserved between Drosophila and mammalian PAT-family members. We ...
PGCsi3.0含有绿色荧光蛋白(GFP)编码基因,有助于检测细胞转染的效率。利用Lipo 2000将PGCsi3.0-LSD1-siRNA转染结肠癌SW620细胞,48 h后于倒置荧光显微镜下观测,转染效率在80%以上( 图1 ),适于进行后续的RNAi干扰实验。 展开阅读 图1 利用倒置荧光显微镜下观察SW620细胞的质粒转染效率 A:一般光源下的SW620细胞;...
We found that LSD1i (ZY0511 or SP2577) treatment and LSD1 knockdown by siRNAs targeting LSD1 resulted in substantial suppression of HR in A2780 and ES2 DR-GFP cell models, and this suppression was similar in extent to that seen with siRNAs targeting two key HR genes, BRCA2 and RAD51....
0 with high purity,THM plasmid-transfected 293 T cells appeared green fluorescence,which was eukaryotic expression vector of GFP green fluorescent protein,suggesting that transfection was successful. The protein expression level of LSD1 was decreased and its main substrati...
7B). To directly elucidate the effects of Id2 on the binding of Tcf3 to the Slamf6 promoter, chromatin immunoprecipitation (ChIP) assays were conducted in GFP-Id2WT and GFP-Id2ΔHLH Jurkat cells. We found that deletion of the HLH domain in Id2 dramatically enhanced the binding activity ...
The expression of GFPL and GFP was measured by immunoblot (B) and real-time qPCR (C). The ratios of GFPL over GFP protein in different samples from five repeats for sh-LSD1 and two repeats for sh-AGO2 were calculated and the ratio in sh-Ctrl sample was considered as 100% miRISC ...
D. GFP 查看完整题目与答案 SDH 板激光器发送失效产生 TF 告警,SDH 板红灯()次/秒。 A. 1 B. 2 C. 3 D. 4 查看完整题目与答案 OTN(光传送网)包含光层子架和()子架。 A. 电层 B. 物理层 C. 数链层 D. 网络层 查看完整题目与答案 电启动的油机发电机组,每次启动按下...
02月06日 10:16 【#185名德国演员集体出柜#】据外媒,185名德国演员联名在《南德意志报》发布一封宣言集体出柜,呼吁增加德国影视戏剧行业的LGBTQ+多样性。宣言中写道:“我们和别的人一起,身份认同为女同、男同、双性恋、跨性别者、酷儿、间性人、非二元性别者。而在此之前,我们无法在不需担忧职业生涯受到影响的...
) Oct4-GFP and alkaline phosphatase positive iPS colonies obtained from reprogramming of B cells (OSKM alone without C/EBPα pulse) treated with S2101 or DMSO as a control. Error bars indicate s.d. (n = 3 biologically independent samples). Statistical significance was determined using a two...
YAP transgenic (YAP) mice were treated with AAV8-GFP or AAV8-Alb>Oaz1. Five days later, 50 mg l-1 Dox was added in drink water to induce YAP overexpression for 10 days. b, Representative liver images of mice described in (a...