LRP5 Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注...
低密度脂蛋白受体相关蛋白1(LRP1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Standards or samples are then add...
HeLa cells were subjected to SDS PAGE followed by western blot with 24899-1-AP (LRP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.View All Images (8) IHC staining of mouse lung using 24899-1-AP Immunohistochemical analysis of paraffin-embedded mouse lung tissue...
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For whole-mount and cross-section immunofluorescence, tissues were incubated with 0.1% Triton X-100 and blocked with 10% donkey serum for 2 h at RT before the application of the primary antibodies. The tissues were incubated with a rabbit anti-LRP1 monoclonal antibody (1:100, Invitrogen MA...
Data were analyzed by unpaired two-sided t-test. NS, not significant; *P < 0.05, ***P < 0.001 Full size image Discussion In this study, we investigated the role of LRP1 in α-Syn uptake and spread. We found that LRP1-deficiency significantly reduced neuronal α-Syn and tau ...
Plates were washed, incubated for 30 minutes with an appropriate secondary antibody, and developed with TMB substrate (KPL Laboratories). Histology and immunofluorescence (IF) For histology, tissues were fixed and inactivated in 4% PFA prior to removal from the BSL-3 lab. Tissues were incubated ...
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, blocked with 5% BSA, and then incubated with the LDLR antibody (ab30532, Abcam) overnight at 4 °C. The cells were then washed, incubated with the secondary antibody (goat anti-rabbit IgG Alexa488) for 1...
Bar graph showing the proportion of LRP1 at the PM of MIO-M1 cells (relative to unstimulated cells) after stimulation with α2M* (60 nM) for 30 min. LRP1 cell surface levels were measured using a cellular ELISA for detection of PM antigens in non-permeabilized cells (antibody anti...
3D using anti-FLAG M2 antibody, and a horseradish peroxidase-conjugate secondary antibody as described under “Experimental procedures”. The amount of ADAMTS-5 or TIMP-3 bound to the cluster II was expressed as a % of the amount of ADAMTS-5 or TIMP-3 bound to the cluster II in the ...