12. Thus, we assessed pulmonary IKKβ protein expression in the lungs of neonatal male and female mice following exposure to systemic LPS. Consistent with previous reports, we found no differences in baseline IKKβ expression
However, the mechanisms involved in the initial formation of innate immune memories are not as well characterized in microglia and hence form the major focus of this study. Here, we used a mouse model of repeated LPS to identify how gene expression, cytokine expression, and microglia morphology ...
(C) Residues that form the binding pocket of RUS are indicated. (D) Microscale thermophoresis (MST) assay was performed to detect the binding kinetics of RUS and blebbistatin (BLE) on full length NMMHC IIA. To further investigate the specific domain of NMMHC IIA that RUS acts on, we ...
[exec] Run with --info or --debug option to get more log output. Run with --scan to get full insights. [exec] [exec] * Exception is: [exec] org.gradle.api.tasks.TaskExecutionException: Execution failed for task ':packageRunCheckFormat'. ...
et al. Liver-resident memory CD8+ T cells form a front-line defense against malaria liver-stage infection. Immunity 45, 889–902 (2016). Article CAS Google Scholar Curbishley, S. M., Eksteen, B., Gladue, R. P., Lalor, P. & Adams, D. H. CXCR3 activation promotes lymphocyte ...
After the infusion, blood was collected via abdominal aorta of each animal and anticoagulated with heparin (20 unit/ml blood). The plasma was isolated by centrifugation. Fifty microliters of plasma or standard was incubated with 50 μl capture beads for 1 h at room temperature, and then mix...
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Because D-alanine in the glycerol phosphate repeating unit of the LTA structure is also critical for its immunostimulatory activity22, we examined whether de-alanylated Sa.LTA affected the inhibition of LPS-induced B cell proliferation. De-alanylated Sa.LTA did not inhibit LPS-induced B cell ...
0.415 × 0.415 × 0.524 μm3with the 405 nm and 633 nm lasers with a pinhole of 42.6 = 1.47 Airy unit (1 μm section). Digital gain DAPI: 1, gain master 860. Offset − 2. A647, Digital gain: 1, Gain master 846 and offset − 7. The morphological feature...
Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is ...