Conclusion:Arnebia euchroma polysaccharides may inhibit LPS-induced TNF-α expression in LPS-stimulated PBMC via down-regulation of MAPK signaling pathway .%目的:研究紫草多糖对LPS活化外周血单个核细胞(Peripheral blood mononuclear cells,PBMC)表达TNF-α,IL-10,IL-18相关因子的影响及其可能的信号通路.方法:...
LPS-stimulated PBMC were cultured in the presence of the flavonoids and TNF伪, IL-1尾 and IL-6 were measured in the supernatants. In parallel, metabolic activity of the PBMC was determined by measuring succinate dehydrogenase activity. Apigenin, chrysin and luteolin dose-dependentl...
LPS刺激的PBMC中的单核细胞产生了所有三种促炎症细胞因子(IL-1β、IL-6和TNF-α),占用mTORi预处理的单核细胞总数的31.4%,而仅暴露于LPS的对照细胞中只有0.8%(图1e)。这些数据显示,用mTORi预处理体外LPS刺激的单核细胞,会导致多能性的强劲增长,特别是IL-1β和IL-6的产生(图1c-e)。 实验结果2 抑制mTOR...
结论:紫草多糖可能是通过抑制PBMC 的MAPK 信号通路进而抑制LPS 诱导的炎症相关因子TNF -A 等高表达。[关键词] 紫草多糖;TNF -A ;IL -18;MAPK;外周血单个核细胞 Arnebia euchroma polysaccharides inhibit expression of inflammation -related cy -tokines in LPS -stimulated PBMC LIU Chun -Hong,WANG Wei,WANG...
Effects of alpha-glucans from Agaricus bisporus on ex vivo cytokine production by LPS and PHA-stimulated PBMCs; a placebo-controlled study in slightly hypercholesterolemic subjects. Eur J Clin Nutr 2010;64:720-6.Volman JJ, Mensink RP, van Griensven LJ, Plat J. Effects of α- glucans from...
CD14+ monocytes were isolated by magnetic cell separation using CD14 beads from PBMC. Monocytes were incubated in media or stimulated with 500 ng/ml LPS for 1 and 3 days, IL4/GMCSF or MCSF for 6 days. Post incubation, monocytes were stained with cell surface antibodies to various ...
In the PBMC from the same donor, sTNFR highly decreased LPS-stimulated PCT (control 10.0%, LPS 27.2%, sTNFR 12.3%), but it did not significantly affect PMA-stimulated PCT. For sepsis patients, PMA stimulated more PCT than LPS ... HJ Choi,MS Cho,YJ Lee 被引量: 0发表: 2006年 加载...
Moreover, although rhIL-1β induced a sustained cytokine production (p < 0.05), ST2825 did not show a significant effect in the secretion of neither pro- nor anti-inflammatory cytokines in rhIL-1β-stimulated PBMC. 展开 关键词: MyD88 inhibition PBMC ST2825 cytokine downregulation ...
Interleukin-1 receptor antagonist (IL-1ra) gene expression in lipopolysaccharide (LPS)- stimulated human peripheral blood mononuclear cells (PBMC): New England Medical Center and Tufts University School of Medicine, Boston, MA 02111Show moreShow less...
Figure 2. CDN inhibits NLRP3 inflammasome activation in primary BMDMs and PBMCs. (A) LPS-primed BMDMs were treated with various doses of CDN and then stimulated with nigericin. Western blot analysis of IL-1β (p17), caspase-1 (p20) in SN and pro-IL-1β, caspase-1 (p45), NLRP3, ...