Sigma-Aldrich 货号 LPS25 包装型号 1 MG 规格纯度 参考价格 3002.41元*本价格含增值税费 服务 √原厂保证 √包邮 √增值税票 数量 -+ QQ微信 添加到订购单查看订购单 产品名称: LPS,E. ColiO111:B4 LPS,大肠杆菌O111:B4 产品介绍: 产品说明
All the common chemicals are of analytical purity and supplied by Merck KGaA, Darmstadt, Germany. All the lipid samples had been supplied by Avanti Lipids (Alabaster, AL, USA). 2.1. Peptide Synthesis The peptides were prepared from 2-Chlorotrityl chloride resin and Fmoc-Trp(Boc)-OH as the ...
Cells were lysed in RIPA lysis buffer containing protease inhibitors (cOmplete protease inhibitor cocktail, Sigma-Aldrich). The lysates (equal amounts of protein) and Laemmli sample buffer were mixed and boiled for 2 min before application to the gel (Bio-Rad, Hercules, CA, USA). ...
coli LPS (strain O55:B5) were from Sigma-Aldrich (Merck, Milan, Italy), while RPMI 1640 culture medium was provided by Corning (New York, NY, USA). The antibodies used were mouse monoclonal anti-GS (BD, Franklin Lakes, NJ, USA), rabbit polyclonal anti-LC3BI/II, rabbit polyclonal ...
The protein concentration was determined using a BCA protein assay (Sigma-Aldrich, Vienna, Austria; 71285-3) according to the manufacturer’s instructions. A total of 20 µg of protein per sample was reduced and alkylated using TCEP and IAA and digested successively using Lys-C (FUJIFILM ...
NO production in RAW 264.7 cells was assessed with the Griess reagent comprising 0.2% naphthylethylenediamine dihydrochloride (Sigma-Aldrich, St. Louis, MO, USA) and 2% sulfanilamide (Sigma-Aldrich, St. Louis, MO, USA) in 5% phosphoric acid (Sigma-Aldrich, St. Louis, MO, USA). Cells were...
Unless indicated otherwise, chemicals were purchased from Carl Roth (Karlsruhe, Germany), Sigma-Aldrich (Seelze, Germany), Thermo Fisher Scientific (Schwerte, Germany), or Merck Millipore (Darmstadt, Germany). Five commercially EVOOs available in German supermarkets were used as a representative of ...