最近的研究表明,IL-4 priming可增加 NF-κB-p65 的结合,从而使 IL-4 极化的 BMDMs 在暴露于脂多糖(LPS)时产生 “扩展协同作用 ” 。由于作者观察到体内 IL-4 在 BL/6 TRMs 中的激活增强了与 NF-κB 和 IRFs 等 SDTFs 相关的 motif 的可及性,作者假设 IL-4 引物和 LPS 暴露之间的协同作用在 BL/...
WAS inhibited the production of IL-1β, but not IL-6, in response to adenosine triphosphate (ATP) and monosodium uric acid (MSU) crystals in LPS-primed BMDMs. Cleavage of caspase-1 and IL-1β was also reduced by WAS. We finally evaluated the in vivo anti-inflammatory effects of WAS ...
文献中所提的LPS-primed bone marrow-derived macrophages(BMDMs）与商业买的巨噬细胞RAW264.7细胞株有...
We have previously shown that low concentrations of FCS are needed for heme to stimulate LPS-primed BMDMs to IL-1β secretion and cell death [48]. We thus tested whether serum proteins could replace the effects of FCS in this model. A low serum concentration (1%) enabled heme to stimulate...
Exo could inhibit the proliferation of primed macrophages and accelerate the polarization of M2 macrophages in vitro, after labeling LPS pre-Exo with a red fluorescent dye (PKH26), they were co-cultured with macrophages to observe whether the LPS pre-Exo would be internalized by BMDMs or not....
Exo could inhibit the proliferation of primed macrophages and accelerate the polarization of M2 macrophages in vitro, after labeling LPS pre-Exo with a red fluorescent dye (PKH26), they were co-cultured with macrophages to observe whether the LPS pre-Exo would be internalized by BMDMs or not....
Accordingly, we pre-primed B6 and Zbp1−/− BMDMs with LPS for 4 h prior to the addition of LPS/5z7, and observed upregulation and cleavage of pro-IL-1β that was dependent on ZBP1 (Fig. 2g). Furthermore, LPS/5z7 treatment induced cleavage of inflammatory CASP1, which was ...
Bone marrow-derived monocytes (BMDMs) were isolated from mice (Suppl. Fig.1) and dosed with PBS, low-dose LPS (100 pg/mL), or high-dose LPS (1 µg/mL) for a 5 day period as we performed previously58,59. Persistent challenges with low-dose LPS enabled the generation of inflammat...
Live O. tsutsugamushi activates caspase-1 in LPS-primed BMDMs in caspase-1- and ASC-dependent manners.JungEun, KooHyeJin, HongAndrea, DearthKoichi, S. KobayashiYoungSang, Koh
RESULTS ATP treatment of LPS-primed TREK-1-inhibited and -deficient BMDMs exaggerated caspase-7 and caspase-11 activity compared to wt BMDMs, but both were dampened with co-treatment with Pannexin and P2X inhibitors. Both TREK1 inhibition and deficiency caused elevated potassium efflux after short...