The purpose of this study was to investigate whether losartan (LOS), an AT1 receptor antagonist, reduces lung damage by inhibiting the induction of high mobility group box 1 (HMGB1) protein and cytokines by lipopolysaccharide (LPS; serotype: O127:B8) in a rat model. Methods We used male ...
Mice were intraperitoneally administered 200 µl of phosphate-buffered saline (PBS) for control group (n = 6) or varying concentrations of LPS (Escherichia coli O127:B8; Sigma Aldrich, St. Louis, MO, USA, L3129) at GD 15.5. The LPS groups were further divided into three groups ...
LPS from Escherichia coli serotype (O127:B8), ethanol, methanol; Folin Ciocalteau reagent; formic acid; gallic acid; sodium carbonate solution, fetal bovine serum and penicillin/streptomycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The trypan blue; TRIzol reagent; SYBR Green kit...
After random selection of healthy pups (controls) and pups exposed to LPS (Escherichia coli, serotype 0127:B8, Sigma, Darmstadt, Germany), the young animals of both substrains were killed on days postpartum (dpp) 7, 10, and 14 to investigate their lungs. Deep anesthesia was achieved by in...
SPC56EC70L8B8EEX、STM32H7A3IGT6、STW81200TR、74VHCT04ATTR、M88142Y-15T1T、SM4T35AY、74VHCT373AT、AN4662、STA1085ELA3、LD1117S50、SD57120、SPC582B50E3L00X、LD29150P18、5962F0152701VXA、1.5KE36ARL、ST62T25CB6、L78M18、STPA003HSD-48X、STM32F732IET6、SPC564B74L8C80EY、LK112M32TR、...
and their mode of linkage determine the serological O specificity of respective strains. They are the main determinants of the classifications of the serotypes of Salmonella species. The diversity of O chains in Enterobacteriaceae may have developed during evolution to allow enteric bacterial to escape...
(LPS,来源于Escherichia.coliserotypeO127:B8)为美 国Sigma公司产品;新生牛血清购自郑州佰安生物工 程有限公司;胰蛋白酶购自华美公司;vWF的ELISA 检测试剂盒购自上海太阳生物公司;兔抗人ICAM- 1多克隆抗体、TNF-原位杂交试剂盒购自武汉博 士德生物工程有限公司;羊抗兔IgG-FITC购于北京 中山生物工程有限公司;卡托...
?TNF-α浓度梯度(1,10,20,50,100ng/ml)购自peprotech LPS(O127,B8/O55,B6都试了)...
HSMZ-C11O、RT0603BRD0753K6L、RT0603BRE071K21L、RP107N331D-TR-FE、LPS3010-223MEC、MB9AF314NABGL-GE1、RT0603BRD07931RL、LPV542DNXT、RT0805BRD07127RL、HT32F0008、RT0805BRD07187RL、MB9BF512NPMC-G-JNE2、RT0603BRE074K22L、HR34B-12WLPE-10S、RT0805BRD0762RL、RT0805BRD0713K7L、RT0603...
(TE1)、SS310-NL、TZMC5V1--、MEC50U01G4、TLK2541PFPR、MAX15101EWL+、MPS1532DR、X7251D、PEB83000EV1.3、T491C107K006AT、127-470M、LM715AMH/883、GP10GE-6329/23、FSUSB74MPX、SC426851P、RFT-3100(CD90-24520-3TR)、Z8S18010VEC、VT1105SCR、PAL16R4ANC、MC14002BAL、PV606BA、AX88790L...