2. 诱导小鼠脾脏B细胞分泌IL-62 3. 诱导大鼠骨髓源性MSCs中IL-6和生长因子的表达3 4. 诱导硬骨鱼巨噬细胞中TNFα和IL-1β的表达4 5. 研究猪瘟病毒感染后猪组织中TLR4的mRNA表达5 LPS-EB来源于E. coli 0111:B4 特点: E. coli 0111:B4...
LPS-B5 from E. coli 055:B5 特点: - E. coli 055:B5菌株是LAL试验中经常用作内毒素标准品的原型内毒素 -高致热原性 -常用于体外细胞活化 应用实例: -在小鼠巨噬细胞中产生促炎性细胞因子分泌13 -诱导小鼠脾脏B细胞分泌IL-614 -诱导大鼠骨髓源性MSCs4中IL-6和生长...
Immunology Evaluation of Porcine IPEC-J2 Cell Line Growth Rate and Immune Response to E. coli (0111|B4) LPS UNIVERSITY OF ARKANSAS Charles Maxwell WangXiaofanThe objective of this study was to evaluate the intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell line as a model to study ...
In the present study, we found that GrA binds the same Gram-negative bacteria as GrK (Figure 6),13 whereas GrA does not bind to LPS (E. coli B111:O4; Figure 6). Whether granzyme binding to Gram-negative bacteria depends on the LPS subtype or is driven by other bacterial molecules ...
・ 大肠杆菌LPS(E.coli0111:B4)、BSA购自Sigma公司; 6 硕士学位论文 第三章材料与方法 1.5.主要仪器 ’・ 低温高速台式离心机(美国ICE公司) ’0-低温高速离心机(湖南湘仪卜,j .. ,・ -C02细胞培养箱(美国SHELL。LAB公司) ・ 超净工作台(北京半导体设备厂》 ...
InvivoGen LPS-EB is a preparation of lipopolysaccharide (LPS) from the Gram-negative bacteria E. coli 0111:B4. LPS-EB is an endotoxin that potently stimulate the immune system via TLR4. InvivoGen LPS-EB is tested on hTLR4 cell-based assay for potency and
Even though we observed a significant (P < 0.05) increase after TRAP stimulation (23.92 ± 6.67%), we did not observe significant modulation after E. coli LPS incubation (6.58 ± 1.19%; data not shown), nor CD14 on platelet membrane. This discrepancy with the observation ...
给大鼠灌胃安慰剂或YMJD,连续14天。末次给药后2 h,气管内注入内毒素(5 mg/kg,E.Coli 0111:B4,Sigma-Aldrich)建立ALI模型。分别于给药后第1、4、7、10、13天记录大鼠体重。内毒素刺激12h后,各组大鼠禁食12h后采集血液、支气管肺泡灌洗液...
MG Lei,Nilofer Qureshi b d,David C. Morrison a c d 摘要: Using a photoactivable, radioiodinated lipopolysaccharide probe, [ 125 I]ASD-LPS (derivatized from purified E. coli 0111:B4 S-LPS), we earlier reported the presence of a 73-kDa (p73) predominant LPS-binding protein on mouse ...
Chemical structure of curcumin (A), resveratrol (B) and sulforaphane (C). Full size image Materials and methods Materials LPS (Escherichia coli 0111: B4; Cat No. L2630) and curcumin (C21H20O6; ≥ 94%; Cat No. 458-37-7) were purchased from Sigma Chemical Co. (St. Louis, MO, ...