For MC3-LNP-IV, lipid components (50% Dlin-MC3-DMA, 10% DSPC, 28.5% cholesterol, 1.5% DMG-PEG, in mol%) were dissolved in ethanol, and RNAs (Cas9 mRNA, hEx45 sgRNA #1, and hEx45 sgRNA #23) were dissolved in 50 mM sodium citrate (pH 4.0). LNP-mRNA and LNP-sgRNA were ...
In the measurement of this doublet AFR spec- trum recorded at 60 s after the mixing, its signal intensity was 5.7-fold greater than that for the original AFR in 2125 mM sodium ascorbate aqueous solution (Fig. 4b). This mixture of Asc and PQQ also gave the AFR with enough amount...
The TG–DSC curves of low–nickel matte mix with or without sodium sulfate samples were carried out with a heating rate (5 °C/min) from 50 to 1000 °C. The results are shown in Fig. 4. sTuGlf–aDte.STChcehreaarraecttherreiezaextoiothne.r mFicigpueraek 4sainshtohwesp...
Treatments significantly affected the ratios of total carbon (C) to total N (C:N ratios) of coral colonies (2-way ANOVA,F = 15.756,p < 0.001). Nitrate eutrophication for 15 days alone did not affect C:N ratios (Fig.5a and Table1). After additional warming, colonies exposed t...
In brief, HeLa cells (ATCC CCL-2), F31 and ∆ fibroblast cell lines37 were cultured in DMEM (4 mM L-glutamine, sodium pyruvate, 4.5 g/L glucose; GE, Logan, UT) plus 5% FBS + 5% FCS with pen-strep (1%). Sub-confluent, cells were collected by trypsinization, ...