The change in fucosylation has previously been investigated using crossed affinoimmunoelectrophoresis, high-pa. anion exchange chromatography, and lectin ELISA. This study describes a surface plasmon resonance-based affinity biosensor assay for quantification of the fucosylation of AGP. Diluted EDTA plasma ...
LDL agarose electrophoresis was performed as described earlier (De Graaf et al, 1991), using Paragon lipo gels (Beckman, Fullerton, California). Data Analysis and Statistics The computer program ASTUTE (Microsoft Inc., Redmond, Washington) was used for the analysis. Data are presented as means ...
The effect of very low protein diets supplemented with branched-chain amino acids on fecal bacterial community at phylum and genus levels. (A) The relative abundance of bacterial community composition at phylum level in fecal samples of pigs fed with very low protein diets supplemented with branched...
Ultrasensitive detection of CBQCA-derivatized amino sugars, amino acids and low molecular weight peptides by capillary electrophoresis has been reported. In capillary electrophoresis, the sensitivity of amine detection of the laser-induced...
Two-dimensional fluorescence difference gel electrophoresis Protein extracts were prepared from mouse brain tissues as described previously70. Proteins were labelled using a CyDye DIGE Fluor minimal labelling kit (GE Healthcare) according to the manufacturer’s instructions71. Briefly, Cy2 was used to ...
Transcript levels are measured by reverse transcription (RT), PCR, and electrophoresis on an automatic DNA sequencer using fluorescence-labeled primers and direct quantitation of the allele-specific RT-PCR products. The discrimination between the allelic products is based on the use of DNA ...
While all tested strains showed resistance towards ceftriaxone, their resistance levels varied widely between 4mg/L and >128mg/L. An allelic variation of the penicillin binding protein gene pbpB3 was identified as the cause of this difference in ceftriaxone resistance levels. This study is the ...
were screened under low-temperature conditions based on the low-temperature-responsive potatoStMYBgenes family analysis obtained by transcriptome sequencing. By analyzed the protein properties and promoters ofStMYB113andStMYB308and their relative expression levels at different low-temperature treatment period...
Reduced dietary CP levels (-2% or -4%) had a significant negative effect (P < 0.05) on the growth performance and FI of ducks. The more the dietary CP levels were reduced, the more the growth performance was decreased. Table 3. Effects of low-protein diets and protease supplementation ...
Hepatic protein (20 μg) was subjected to NuPAGE 5–10% Bis-Tris gel electrophoresis and transferred to polyvinylidene fluoride membranes (0.45 μm), which were subsequently blocked with 5% milk, incubated overnight at 4 °C with primary antibodies against CPS1 (Santa Cruz Biotechnology...