Polyzwitterionic biomaterial with pH-responsive and hemocompatible dual functions is formulated by the copolymerization of 2-(N, N-dimethyl amino) ethyl me... Antoine,Venault,Chun-Wei,... - 《International Journal of Polymeric Materials & Polymeric Biomaterials》 被引量: 4发表: 2015年 Synthesis,...
To demonstrate the colloidal stability of the NP-Pep, we determined the zeta potential (ζ-potential) of the NP-Pep suspended in 0.1× PBS buffer (pH 7.4) to be ~−38 mV, which has been previously shown to be both ideal for serum protein applications and sufficient for electrostatic ...
We performed our single-molecule FRET experiments with three dual-labeled constructs of FUS-LC varying the intramolecular distance (N-to-86, N-to-108, and N-to-148). Under the solution condition (pH 7.4, 250 mM NaCl), FUS-LC remains monomeric up to a concentration of 50 µM as ...
Three extraction buffers were used and their total protein extraction capacity from the tissue was evaluated. We then analyzed and estimated the total protein concentration and antibacterial capacity of obtained extracts. Aiming to isolate the bioactive peptide that confers antimicrobial activity, the ...
Possibly, this could result from an interaction of these repeats with a β-propeller domain also present on LRP1 that has been observed for the LDL receptor at low pH (42) or from the dimerization of LRP1, which is known to occur (44). These results stress the importance of examining ...
It is possible that the high ionic strength used for isolation of lipoproteins with KBr and NaI could alter the pattern of associated exchangeable proteins. Here we describe lipoprotein classes fractionation from up to 0.5 ml of serum or plasma with buffers of physiological ionic strength and pH ...
The following protocol was used to screen for specific antigen (β-galactosidase) in the coliphage λ system. Buffers: PBS: 8 g NaCl; 2.17 g Na2HPO4.7H2O; 0.2 g KH2PO40.2 g KCl; water to 1 liter. PBS: 10 mM Tris-HCl, pH 7.5; 0.1M NaCl; 10 mM MgCl2; 0.05% gelatin. ...
A variety of buffers may be used for the dilution of fibrinogen-containing component. In some embodiments, the buffer comprises at least one of sodium chloride, tri-sodium citrate dihydrate, glycine, arginine hydrochloride and calcium chloride dihydrate. In some embodiments, the buffer comprises about...
Biochemical characterization. Purified rInvHJ14 had activity in the range of pH 7.0–9.5 with an apparent pH optimum of 8.0 (Fig. 4a). Over 85% of its initial activity was observed after incubating the enzyme for 1 h in buffers with pH 7.0–9.0 (Fig. 4b). The apparent ...
The reaction was maintained at pH 4.8 with 0.01 M HCl. After 1 h, sodium acetate to a final concentration of 0.5 M was added to quench the reaction, and the solution was stirred for an additional 1 h. UV scanning from 190 to 260 nm was performed to determine the ...