Figure 3: Increased transcript detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq v4 Ultra®Low Input RNA Kit for Seq...
cDNA Synthesis KitRNA-Seq Library Preparation SMARTer Universal Low Input RNA Kit for Sequencing (Cat. Nos. 634938, 634940) Ion Torrent or Illumina kits (not included) SMARTer Universal Low Input RNA Library Prep Kit (Cat. Nos. 634945, 634946)Low Input Library Prep Kit included (for Illumina...
Hieff NGS® Single Cell/Low Input RNA Library Prep Kit是兼容Illumina®和MGI®双平台,可以对1~1000个细胞或者10 pg~10 ng的总RNA进行测序文库构建的试剂盒。本试剂盒可以进行全长cDNA的合成,完成全长cDNA的富集,然后对获得的全长cDNA扩增产物进行酶切法文库构建,与传统的建库方法比较,本品采用高质量的片...
RiboGone-Mammalian试剂盒特别适用于随机引物从DNA合成的,RNA-seq的或者转录组分析的样本准备。采用 SMARTer Stranded RNA-Seq Kit 或者 SMARTer Universal Low Input RNA Library Prep Kit进行建库,残留的rRNA序列在1-5%之间,甚至更低。特点:这是一种有效去除rRNA的方法,对于人,小鼠和大鼠样本去除率超过95%对于任何...
Figure 3. SSmRNA + UMIs combined with Cogent NGS data analysis tools are a complete solution for full-length transcriptome analysis and RNA counting for single-cell and ultra-low-input RNA-seq.The kit's workflow goes from cDNA synthesis to library prep. Sequence...
As described previously24,25, small RNA sequencing of the 24 samples/libraries was performed using the NEXTflex small RNA-seq kit v3 (Bio Scientific, Austin, TX, USA). The adapter-dimer reduction technology incorporated into this kit allows low input library preparation. Briefly, 100 ng total ...
Fig. 1: Summary of low-input library preparation methods, project design, and schema of LILAP. aComparison of low-input methods on the PacBio sequencing platform. “DNA input” pertains to the currently commonly employed PacBio Sequel II platforms. PB Template prep kit is used to prepare the...
二代测序文库制备 KK8484 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (96rxns) 98000 96rxn二代测序文库制备 KK8500 KAPA Hyper Prep Kit (8 reactions) 3100 8 rxn二代测序文库制备 KK8501 KAPA Hyper Prep Kit, no amplification (8 reactions) 2970 8 rxn二代测序文库制备 KK8502 KAPA Hyper Prep ...
Transcriptome sequencing library preparation According to manufacturer instructions, total RNA was extracted using RNA prep Pure Plant Kit (Tiangen Biotech-Beijing Co. Ltd). A volume of 1 μg RNA per sample was used as input material for the RNA sample preparation. Following the manufacturer's rec...
Library Kit DNA Input Total Number of Sequencing Reads Per Library Staphylococcus aureus E. coli K12 NxSeq® AmpFREE Low DNA Library Kit 75 ng 5,649,946 4,305,882 Kapa Hyper Prep Kit 250 ng 4,838,726 (-15%) 1,647,452 (-62%) Illumina TruSeq DNA PCR-Free Library Prep Kit 1 ...