we next sought to modify this system for use inC.difficilevia replacement of the ParaEpromoter controlling sgRNA expression. Accordingly, the promoter region oftcdBwas PCR amplified fromC.difficile630 genomic DNA using primer pair PtcdB_XbaI and PtcdB_SalI and cloned betweenXbaI...
README License Note Due to the adjustment of the /boot/vmlinuz-xxx file in the kernel from 32MB to 64MB, Amlogic series boxes must manually update the OpenWrt system bootloader, otherwise, the OpenWrt system will fail to start after updating the kernel. To manually update, download the updat...
Under a Creative Commons license open accessClostridioides difficile is the primary cause of antibiotic-associated diarrhea and colitis, a healthcare-associated intestinal disease resulting in a significant fatality rate. Colonization of the gut is critical for C. difficile pathogenesis. The bacterial ...
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to ...
Utilizing unique carbohydrates or utilizing them more efficiently help bacteria expand and colonize new niches. Horizontal gene transfer (HGT) of catabolic systems is a powerful mechanism by which bacteria can acquire new phenotypic traits that can incre