The Hunt for Lost License Plates.Focuses on the contest sponsored by the magazine 'Motor Trend' in 2000, which involves finding automobile license plates buried in the Death Valley in California. Significance of the use of Global Positioning System; Winners of the contest.Tune...
License Plates Other Home How to Replace a Lost Car TitleHow to Replace a Lost Car Title Drivers with a lost car title are generally required to initiate a replacement procedure timely and properly, thus staying in accordance with state road rules and regulations. Ordering a duplicate car titl...
Brian Pearson was stopped by police in 2015 while his brother was dropping him off at the train station for work. The cops told the men they smelled marijuana, but Pearson, who is Black, believes his race played a role. "I guess they'd seen two Black guys and Pennsylvania plates," he...
A total of 120 μL of the transformed cells were then plated on ampicillin agar plates and incubated between 12–18 h at 37 °C. A minimum of 8 clones per plate were chosen, and plasmid DNA were generated using the boiling lysis method. Both the forward and reverse strands were ...
Lysates were immediately used for a competitive immunoassay (Direct cyclic AMP Enzyme-linked Immunosorbent Assay kit, Enzo Life Sciences, Farmingdale, NY, USA), to measure the intracellular concentration of cAMP. Data were analyzed by using Microplate Manager 4.0 software (Bio-Rad, Hercules, CA, ...
Fresh colony material was spotted on target plates and covered with 1.0 μl of α-cyano-4-hydroxy-cinnamic acid (HCCA) matrix solution (Bruker, Billerica, Massachusetts). After drying, the protein profiles were analyzed using a Microflex™ LT MALDI-TOF System, the Maldi Biotyper RTC and ...
Mice were infected using a widely used murine CDI model22with minor modifications. Briefly, 6-week-old female C57BL/6 mice from Taconic Bioscience (Rensselaer, NY) arrived at the University of Colorado on day 7 of the experiment. During experiments, mice were cohoused in groups of 4–5 mic...
[36] revealed by STEM-EDX analysis of the precipitates in the 718Plus alloy that Nb can also be present in η phase plates. Tan et al. [37] suggested that when the Ti/Al ratio in the liquid phase is higher than around 2.0, the η phase is favorable to form; however, when the ...
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Maniatis, T.; Fritsch, E.F.; Sambrook, J.Molecular Cloning: A Laboratory Manual; Cold Spring Harbor; Cold Spring Harbor Laboratory Press: New York, NY, USA, 1982. [Google Scholar] Trieu-Cuot, P.; Carlier, C.; Poyart-Salmeron, C.; Courvalin, P. A pair of mobilizable shuttle vecto...