License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made....
In this communication, we describe the organometallic hybrid antibiotic 2c, in which the drug metronidazole is connected to a gold(I) N-heterocyclic carbene warhead that inhibits bacterial thioredoxin reductase (TrxR). Metronidazole can be used for the treatment with the obligatory anaerobic pathogen ...
F Piscione4, M Hahne10, LJ De Windt*,6, MC Turco2,11 and V De Laurenzi*,2,5 Molecular mechanisms protecting cardiomyocytes from stress-induced death, including tension stress, are essential for cardiac physiology and defects in these protective mechanisms can result in pathological alterations....
In all, 1 Â 106 cells were seeded in a six-well plate, allowed to grow until 70–80% confluency. To measure DNA synthesis as indicator for cell division 25 mg/ml BrdU (from Sigma-Aldrich) was added to the cell culture for 1 h. After washing, free BrdU cells were trypsinated, ...
A minimum of 8 clones per plate were chosen, and plasmid DNA were generated using the boiling lysis method. Both the forward and reverse strands were sequenced with BigDye Terminator v3.1 Cycle Sequencing Kit (Invitrogen, Carlsbad, CA) Analysis of the sequences was done using Sequencher 5.0 (...
Vibrio parahaemolyticusis a Gram-negative, moderate halophile that is ubiquitous in the marine and estuarine environments, present in sediments, shellfish and zooplankton [1,2,3,4]. Consumption of raw and undercooked shellfish is the primary route ofV. parahaemolyticusinto the human host, where it...
Your space vehicle is shaped sort of like a syringe, with a “pusher plate” on the back, connected to shock absorbers, which connect to the main body of the vehicle. You drop a specially designed nuclear bomb out the back. The nuclear bomb explodes, sending a blast of plasma toward the...
very skilled, but Rybicki was by far the more congenial of the two: even-tempered, fair, objective, flexible. Rybicki was a team player and by his own account got along very well with GM president Pete Estes and chairman Tom Murphy. In personality, he was the antithesis of Bill Mitchell...
License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made....
In a 96-well plate, a 50 µL aliquot of the bacterial inoculum was added to a 50 µL aliquot of peptide TtAP-1 to give a final concentration of peptide of 6.25 µg/mL (1× MIC). Wells were prepared in triplicate for each time point (0, 15, 30, 45, 60 min) with a ...