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Strains were grown in TY broth as this is a glucose-free medium that supports toxin (tcdA and tcdB) gene transcription37,38. The recorded OD600 values were plotted on a logarithmic scale (Supplementary Fig. 3 and Supplementary Data 6). Statistical analyses were performed using a 2way ANOVA ...
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Multiple genes encoding structural components of the flagella, including some that have been shown to induce inflammatory responses from host cells in vitro and in vivo, were decreased in expression in wild type (Fig.2b). In contrast to flagellar genes, wild type had increased expression of the...
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