To explore the function ofsox3in ovary development in zebrafish,sox3knockout zebrafish lines were first generated using CRISPR/Cas9 technology. We used a CRISPR design web tool (http://crispr.mit.edu/) to design gRNA targeted the 5′ upstream of coding region (Fig.1A). Two independentsox3m...
because of a non-synonymous polymorphism in the coding sequence of a gene which would modify the corresponding protein structure and function. The defective protein could thus disrupt the molecular cascade that normally leads to the production of sexual females. Eleven of the 32 genes from the cand...
This understanding of the genetic pathways that regulate auditory function has revealed new targets for pharmacological treatment of the disease. Moreover, approaches that are based on stem cells and gene therapy, which may have the potential to restore or maintain auditory function, are beginning to...
Luciferase assays confirmed the loss-of-function effect of these substitutions on the MITF promoter both alone and in the presence of the cofactor PAX3 (Figure S1D). The seven likely pathogenic alterations found in this study are shown along with the main functional domains of SOX10 (Figure ...
ARTICLES OPEN NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease T Schwerd1,2,32, RV Bryant1,3,32, S Pandey1,32, M Capitani1, L Meran4, J-B Cazier5,33, J Jung1, K Mondal6, M Parkes7, CG Mathew8, K Fiedler9,10, DJ McCarthy5,34, WGS500 ...
Transfected hücreleri ve onların kızı hücreleri onların genleri CRISPR kaynaklı mutasyonların tabi iken, onların ayak izleri zamanla kayıp alabilirsiniz. Böylece, genetik etiketleme yaklaşımlar bu sorunu aşmak için uygun olacak. Son zamanlarda onlar...
Also, we do not have a firm basis to explain why the loss of function of a ‘generic’ tumor suppressor such as CABLES1 caused a pituitary-specific phenotype in our patients. We could speculate that the missense variants found in these cases might cause loss of crucial pituitary-specific ...
A GI/M/1/K queuing model has been used to compute the packet drop probability on a given link. The problem of optimizing OSPF weights is known to be NP-hard even for the case of a linear objective function Bernard Fortz and Mikkel Thorup (2000), We use online simulation (OLS) ...
Hayabuchi Y, Ono A, Homma Y, Kagami S (2018) Pulmonary annular motion velocity reflects right ventricular outflow tract function in children with surgically repaired congenital heart disease. Heart Vessels 33:316–326 Article PubMed Google Scholar Blue GM, Kirk EP, Giannoulatou E, Sholler GF...
We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some...