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Illumina libraries were prepared using the NEB Ultra II DNA library kit using the manufacturer’s protocol. We used 4–5 PCR cycles to amplify next-generation sequencing (NGS) libraries and index samples. In situ Hi-C The Hi-C protocol was performed similarly to ref. 4. In brief, fixed ...
The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a ...
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Active-site loops play essential roles in various catalytically important enzyme properties like activity, selectivity, and substrate scope. However, their high flexibility and diversity makes them challenging to incorporate into rational enzyme engineer
functionality in the runloop is pretty objectively harder than adding and maintaining it in "purity land" at a higher level, which is a long-winded way of saying that every time we add a primitive algebraic case toIO, we increase the complexity of the runloop and we should be careful ...
One embodiment of an apparatus utilizing disclosed techniques includes at least one execution unit and a prefetcher utilizing a variable length loop detector to fetch a control sequence for the execution unit. The variable length loop detector is capable of predicting branches for loops having changing...
The first lesson in theGetting Startedguide is to create a basic game loop which creates a Direct3D device, swap chain, and update/render loop. Setup Our starting point is to use theDirect3D Win32 Gameor theDirect3DUWPGameproject template. Install theVS 2019/2022VSIX on your development sys...
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and highdose XP-containing serum(XP-L,XP-M,XP-H) groups for 48 h,cells intervened with no drug rat serum and PD98059[extracellular signal-regulated kinase(ERK) inhibitor]as negative and positive controls(NC and PC)groups.Cell proliferation assay was made using cell counting kit-8(CCK8)....