转染采用pmaxGFP质粒,以PI染色判断细胞活力。数据计算采用多次实验的平均。 2、Lonza电转仪LV单元专用电转试剂盒——提高转染效率和细胞活率,GMP级别耗材 Lonza LV流式细胞电转仪专用转染试剂盒,由转染试剂、补充剂、电击耗材、pmaxGFP阳性对照质粒等组成。 ①电转试剂——根据不同细胞单独优化转染液和补充剂配方,...
相比脂质体转染、病毒转导、传统电穿孔仪,Lonza 2b电转仪操作简单,重复性高,在原代细胞和细胞系中实现更高转染效率、更高细胞活率、更快基因表达,加快推进实验进程。 Fig. Primary NHDF-neo cells were transfected with labeled plasmid DNA encoding GFP, fixed after 2h in 3.5%PFA and analyzed by confocal m...
Lonzagreen fluorescence protein gfp plasmid amplification pmax fp green c vector Green Fluorescence Protein Gfp Plasmid Amplification Pmax Fp Green C Vector, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - sco...
Nucleofector®Solutions and Supplements. These act as a protective environment for high transfection efficiency and cell viability, while maintaining physiologically relevant cellular conditions. Specified Nucleofection®vessels, pipettes, and a fluorescent positive control vector (pmaxGFPTM Control Vector) ...
RBP-JK GFP Reporter Plasmid(RBP-JK-GFP报告基因质粒) 二级品 翌圣生物科技(上海)股份有限公司 1年 查看详情 ￥3365.00/瓶 上海 AP3-Luc荧光素酶报告基因质粒(AP3 Luciferase Reporter Plasmid) 翌圣生物品牌 翌圣生物科技(上海)股份有限公司 1年 查看详情 ￥3365.00/个 上海 SP1-Luc荧光素酶报告基因质粒(SP...
带有Luc-GFP萤火虫酶+绿色荧光双荧光载体: pTRE-neo-GFP-Luc (双荧光,可直接表达,也可慢病毒包装;) pEF1-GFP-Luc (双荧光,可直接表达) pCMV-Luc-EGFP (双荧光,CMV启动子-Luc-SV40启动子-EGFP,可直接表达)启动子增强子报告载体(Promega,带有Luc荧光报告基因): pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-...
Methods: We firstly delivered a 13 kb Cas9-EGFP and a 3.5 kb pMAX-GFP vector into PFFs via 7 programs recommended by the Lonza basic protocol. We then tested 6 customized dual-electroporation programs for delivering the 13 kb plasmid into PFFs. In addition, we screened potential alternative...
cells 2 µg plasmid DNA (in 1 – 5 µl H 2 O or TE) or 2 µg pmaxGFP® Vector or 30 – 300nM siRNA (3 – 30 pmol/sample) 100 µl Cell Line Nucleo ector® Solution V Please make sure that the entire supplement is added to the Nucleo ector® Solution 2.1 ...
2 µg plasmid DNA (in 1 – 5 µl H 2 O or TE) or 2 µg pmaxGFP® Vector or 30 – 300nM siRNA (3 – 30 pmol/sample) 100 µl Cell Line Nucleo ector® Solution T 2.1 Please make sure that the entire supplement is added to the Nucleo ector® Solution ...