Nanopore sequencing has emerged as a novel technique that can analyze long strands of DNA (long-read sequencing) without chemically treating the DNA. Interestingly, nanopore sequencing can also extract epigenetic status of the nucleotides (including both 5-Methylcytosine and 5-hydroxyMethylcytosine), ...
DNA strands of well-defined sequence are valuable in synthetic biology and assembly. Drawing inspiration from solid-phase , here we describe a DNA assembly method that uses time, or order of addition, as a parameter to define structural complexity. DNA building blocks are sequentially added with ...
Molecular recognition between two double stranded (ds) DNA with homologous sequences may not seem compatible with the B-DNA structure because the sequence information is hidden when it is used for joining the two strands. Nevertheless, it has to be invoked to account for various biological data....
The ability for long-range charge transport to occur in double-stranded nucleic acids of different comformations is considered in light of modeling studies that show interstrand base-base overlap between the opposing, complementary strands that make up RNA/DNA hybrid duplexes. Thus, the possibility ...
Folding super-sized DNA origami with scaffold strands from long-range PCR A 26 kilobase single strand DNA fragment was obtained from long-range PCR amplification and subsequent enzymatic digestion, which we folded into a super-si... H Zhang,C Jie,D Pan,... - 《Chemical Communications》 被引...
(dsRNA) species is unraveled and one of the strands is subsequently loaded into a complex containing one of the four humanargonaute proteins(Wang et al., 2009) resulting in the RISC. The RISC mediates the repressive function of the miRNA through partial complementarity to targeted mRNA ...
Transcripts homologous to both of the complementary strands of the Kpn I sequences are present, but with a strong bias towards one strand. 展开 关键词: Humans Hela Cells DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific DNA, Neoplasm RNA, Neoplasm Cloning, Molecular Nucleic Acid ...
but opposite strands of the DNA. These cis-NATs were firstly identified in viruses, then prokaryotes and finally in eukaryotes. In eukaryotes (except nematodes), approximately 5–29% of the transcriptional units are involved in the overlap [51]. Thecis-NATs are transcribed by RNApolymerase II ...
Resolution of Holliday junctions is a critical intermediate step of homologous recombination in which junctions are processed by junction-resolving endonucleases. Although binding and cleavage are well understood, the question remains how the enzymes locate their substrate within long duplex DNA. Here we ...
Transcripts homologous to both of the complementary strands of the Kpn I sequences are present, but with a strong bias towards one strand.doi:10.1038/304277a0Shafit-Zagardo, BridgetBrown, Fred L.Zavodny, Paul J.Maio, Joseph J.Nature Publishing Group UKNature...