Interestingly, in pancreatic cancer, Ral guanine nucleotide-exchange factors (Ral GEFs) have emerged as the key effectors of the K-Ras pathway instead of the B-Raf, extracellular signal-regulated kinase (ERK) or MAPK pathways. As Ral GEFs activate RalA and RalB small G proteins, Lim et al....
The main purpose of pyruvate oxidation is to oxidize pyruvate to create acetyl CoA. Acetyl CoA is an intermediate of the Krebs cycle. Both pyruvate oxidation and the Krebs cycle are essential components of aerobic respiration, the process of converting food into energy for the cell. How many st...
The enzyme from the former sources was dependent upon the presence of acetyl-CoA for full activity; the enzyme from A. nidulans was unaffected by the presence or absence of acetyl-CoA.关键词: Pyruvate carboxylase Aspergillus niger Enzyme location ...
Formation of all three enzymes was inhibited by cycloheximide, but not by chloramphenicol, indicating that they were synthesized in the cytosol. In anaerobically growing cells the levels of acetoacetyl CoA thiolase and HMG CoA synthase were decreased by ergosterol, whereas HMG CoA reductase levels ...
The Krebs cycle, also known as the citric acid cycle or TCA cycle is a series of reactions that take place in the mitochondria resulting in oxidation of acetyl CoA to release carbon dioxide and hydrogen atoms that later lead to the formation of water.
Equal amounts of protein from cell lysates were resolved by reducing SDS-PAGE and transferred to nitrocellulose membranes. Proteins were detected using primary antibodies at a 1:1000 dilution: phospho-AMPK (Thr-172), phospho-acetyl-CoA carboxylase (phospho-ACC), phospho-eIF2α, phospho-GSK3β ...
of carbonyl compounds52,53(Supplementary Fig.7and Supplementary Table3) using the nanoESI-MS platform shown in Fig.1b. It was found that 2-acetylpyridine (121.14 g∙mol−1) was the most suitable reagent for enhancing the intensities of thesn-position diagnostic ions. This reagent was...