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Patients were identified as testing positive if they had at least one RT-qPCR test where the viral load was able to be quantified (≥LLOQ). Six participants were seen to present multiple, sequential, positive RT-qPCR results and were classified as having a sustained infection. Three ...
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’) I still can’t be up and moving around for more than a few minutes without feeling a whoosh of heat in my face and that feeling that something might come up at any minute, or without having a coughing fit so bad it literally takes me a few minutes to find solid...
tuberculosis complex DNA by AccuProbe DNA probe assay and by an in-house multiplex PCR assay, performed as described previously [23, 24]. Phenotypic drug susceptibility testing All M. tuberculosis isolates were subjected to phenotypic DST against rifampicin, isoniazid, ethambutol and streptomycin by ...
qRT-PCR The rats were sacrificed three days after surgery through CO2 inhalation, and the hearts were collected. The muscle tissues of left ventricular in the border zone were dissected and frozen in liquid nitrogen, after which the total RNA was extracted using Trizol reagent (Invitrogen, Grand...
We first detected the BIR-enabled PCR product at ∼2 h after DSB induction, and it further increased to 25% in WT cells by 6 h (Figures 3F and 3G). The appearance of the strand invasion product was strongly reduced in arp8Δ, while in nhp6ΔΔ cells, it appeared after 1 h and...
(LSU). The variable D1 and D2 domains of the LSU rRNA gene were PCR-amplified as a single DNA fragment directly from whole yeast cell suspensions, following the procedure and PCR parameters described by Kregiel et al. (2018). The yeast LSU D1/D2 domain was amplified and sequenced ...
pEGFP-SNX9 dSH3 lacking amino acids 1–67 was constructed using PCR cloning. Mutations in pEGFP-SNX9 that are F208A/F211A and K522A/K528A (ref. 62) were introduced using phusion mutagenesis (Thermo). NATURE COMMUNICATIONS | 8:15873 | DOI: 10.1038/ncomms15873 | www.nature.com/nature...
For PCR analysis, the left and right superior samples were subdivided into tissue directly interfaced with the membrane and control tissue, taking care to discard the midline incision site and the margin around the implant. For control and sham animals, an equal area of control tissue was obtain...