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Transcription and DNA looping appear to stabilize nucleosomes10,18,19 whereas DNA damage repair destabilizes them18. Together, these findings reveal a dynamic interplay between different nuclear processes, chromatin density, and the thermodynamically driven motion of nucleosomes. However, recent reports ...
Therefore, although hydrophobicity at 328 can still play a role in aggregation, the repair effect of I328S is likely specific to the S320F mutant and may be mediated by the disruption of the F320 and I328 interaction. Because hydrophobicity can be important for aggregation, we next tested ...
End repair was immediately followed by resuspending the DNA-antibody-bead matrix with 1mM ATP, 100μM dNTPs, 15U T4 DNA polymerase (NEB), 5U Klenow large fragment DNA polymerase (NEB) and 50U T4 PNK (NEB) in 1X NEB buffer 2 and incubating at 30°C for 30 min. After two RIPA ...
The fat tail of sheep is an important organ that has evolved to adapt to extreme environments. However, the genetic mechanisms underlying the fat tail phenotype remain poorly understood. Here, we characterize transcriptome and lipidome profiles and morph
The zeta-potential of SD-MBG and SD-MBG + BMP-2 in ultrapure water was measured using a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK) instrument equipped with a 4 mW HeNe laser (633 nm) and a light scattering detector positioned at 90◦. Bioactivity Test in Simulated Body Fluid...