The eluate was incubated 3 h with 200 μl high-capacity streptavidin agarose resin (Thermo Fisher Scientific). The beads were washed twice for 15 min in buffer 1 supplemented with 0.05% GDN. Subsequently, bound proteins were eluted for 45 min in buffer 1 supplemented with 2.5 mM...
It possesses numerous outstanding properties, such as high melting point, low thermal conductivity, high surface area, good chemical stability, low cost, and flame retardancy attributed to the capacity of improving the char formation on the burning materials due to its particle shape [3–5]. In ...