Transfection procedures were performed employing Lipofectamine 2000 (Invitrogen, USA), following the manufacturer’s instructions. The assessment of transfection efficiency was conducted 48 h after transfection, utilizing quantitative real-time polymerase chain reaction (qRT-PCR). The target sequences are ...
The protein-coding genome constitutes almost the entire genome of unicellular yeast1; by contrast, <1% of the human genome harbors protein-coding gene sequences and much of the rest is transcribed as non-coding RNA (ncRNA)2, suggesting that the noncoding transcriptome is critical for the comple...
Nuclear and cytoplasmic separation was performed using the PARIS Kit (Life Technologies, USA) according to the manufacturer’s instructions. Fluorescence in situ hybridization (FISH) The FISH assay was performed in MDA-MB-231 cells according to the specifications of the manufacturers. The Cy3-labeled...
USA) as described previously [16]. The extracted RNA was used to synthesize cDNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Japan) according to the manufacturer’s instructions. For miRNAs, reverse transcription was performed using the miRNA First Strand cDNA Synthesis ...
The luciferase activity was measured using Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s instructions and the Renilla luciferase activity was used as a control for normalization. Co-Immunoprecipitation (Co-IP) S2 cells were co-transfected with Cactin-V5 and Dorsal-HisA,...
At the end of the treatment period, total RNA was prepared using TRIzol (Invitrogen, CA, USA), according to the manufacturer's instructions. The BAC clones were obtained from the Rowell Park Cancer Institute BAC Library and BAC DNA was isolated using the the QIAprep Spin Miniprep kit (...
2018-10-22上传 lncrna linc01446 promotes glioblastoma progression by modulating mir-489-3ptpt1 axis.[2018][biochem biophys res commun][10.1016j.bbrc.2018.07.067] 文档格式: .pdf 文档大小: 532.26K 文档页数: 7页 顶/踩数: 0/0 收藏人数: ...
RNA Isolation and qRT-PCR Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA was extracted and retrans- cribed into DNA. The retranscribed DNA was diluted 10 times as a template, and three replicates were set for ...
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Abstract RPA is a master regulator of DNA metabolism and RPA availability acts as a rate-limiting factor. While numerous studies focused on the post-translational regulations of RPA for its functions, little is known regarding how RPA availability is controlled. Here we identify a novel lncRNADisc...