Manassas Park, VA17,54812525.2% Dulles Town Center, VA5,36214342.6% Cities with similar population CityPopulationCost of Living IndexNational Avg. River Ridge, FL5,3621054.6% Three Oaks, FL5,36212121.2% Cut Off, LA5,362936.8% Union Beach, NJ5,36212121.4% ...
--Manassas, VA Shine has been a very easy-to-use curriculum. We rotate lessons through four teachers, so it is helpful to have curriculum that is easy to understand and prepare. We are very happy with Shine! --Seneca, SC We are using and loving The Peace Table: A Storybook Bible and...
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Bacillus strains were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Q5® High-Fidelity DNA Polymerase, restriction enzymes, and NEBuilder® HiFi DNA Assembly Master Mix were purchased from New England Biolabs (NEB, Ipswich, MA, USA). Oligonucleotides for genetic...
Cell culture. U251 glioblastoma cell line was purchased from American Type Culture Collections (Manassas, VA, USA). This cell line was grown as monolayer in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with fetal bovine serum (FBS) at a final concentration of 10%, 2 mM L-...
Manassas (6) Marion (1) Marshall (1) Martinsville (2) Maurertown (1) Mc Lean (1) McLean (2) Mechanicsville (3) Midlothian (4) Montross (1) Montvale (1) Natural Bridge (1) Nelsonia (1) New Market (1) Newport News (12) Norfolk (9) Oakton (1) Onancock (1) Orange (1) Penn ...
CV-1 cells were obtained from ATCC (Manassas, VA) and maintained in 10% fetal bovine serum (Hyclone Laboratories, Logan, UT) and EMEM media. Transfections were carried out by using transfectin (Bio-Rad, Hercules, CA) according to manufacturer’s instructions. The RXR agonist used in this ...
Human embryonic kidney cells 293 (HEK293, ATCC, Manassas, VA) were cultured in phenol red–free Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Waltham, MA) supplemented with 2 mM l-glutamine (Invitrogen, Waltham, MA), heat-inactivated 10% fetal bovine serum (FBS HI, Gibco). Cell ...
The MCF10a, MCF-7 and MDA-MB-231 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA). The MCF10a was cultured in DMEM/F12 medium (Invitrogen Life Technologies, Carlsbad, CA) supplemented with 20 ng ml−1 epidermal growth factor, 100 ng ml...