Picro-Sirius Red staining for liver fibrogenesis.Yong WangBin XiongBin LiangHui ZhaoHui LiJun QianHuiMin LiangGanSheng FengChuanSheng Zheng
Tissue samples were formalin fixed then processed by the Histopathology Core Facility, Charles Perkins Centre, University of Sydney. H&E staining and immunohistochemistry (antibodies; Supporting information3) on 5 µm paraffin sections have been described41. Following 0.1% Picro-Sirius Red (Sigma-...
*P = 0.024) and lobular (n = 4 per group, **P < 0.0001) inflammation as well as fibrosis based on Sirius red staining (n = 6 per group, **P < 0.0001) at P14. Yellow circle: the necrotic area; Yellow arrowhead: acidophilic body; ...
Moreover, in the absence of MMP-12 there is not only a prominence of elastin during fibrogenesis but interestingly other collagenous matrix proteins (as defined by Sirius red staining) also [27]. 9. Functional contribution of individual cell lineages to fibrosis resolution Whilst gene array and ...
Masson trichrome staining and Sirius Red staining were performed to evaluate fibrosis. The steatosis, activity, and fibrosis score was evaluated based on histologic features. Frozen sections were embedded in optimal cutting temperature compound and stained with Oil Red O to analyze lipid accumulation. ...
on liver progenitor cell response and fibrogenesis were assessed in three models of liver fibrosis (carbon tetrachloride, methionine-choline deficient diet, 3,5,-diethoxycarbonyl-1,4-dihydrocollidine diet) by quantitative real time (qRT) PCR, Sirius-Red staining, hydroxyproline assay, and semiquantit...
Material and methods: Paraffin sections of liver bioptic material were used following H+E and Sirius red staining and immunocytochemical detection of cytokeratins 7 and 19. Statistical analysis used StatXact software (Cytel Co.). Results: The intensity of CK7 and CK19 detection was found to ...
The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as f...
14) combined with hematoxylin and eosin (H&E) staining in liver isolated from young (3–4 months) and old (18–22 months) mice (Fig. 1a and Extended Data Fig. 1c). Importantly, Sirius red staining showed no profound increase in liver fibrosis in old livers (Extended Data Fig. 1c)....
Sirius Red staining was carried out using NovaUltraTM Sirius Red Stain Kit from IHC WORLD by following the instruction of the vendor. H&E, IHC, and IF H&E, IHC, and IF staining procedures were similar to those of the previous reports21. Images were captured at ×20 lens aperture and ...