b, H&E staining of one young (upper panel) and one old (lower panel) liver specimen used for spatial transcriptomics (scale bar, 500 µm) and plots showing the expression levels of Glul, Cyp2f2 and Cyp2e1 indicated by color. The color gradient represents normalized gene expression. ...
ArticleLiver receptor homolog-1 (NR5A2) orchestrates hepatic inflammation and TNF-induced cell death Author links open overlay panelRebekka Lambrecht 1, M. Eugenia Delgado 1 3, Vincent Gloe 1 4, Karina Schuetz 1, Anna Pia Plazzo 1, Barbara Franke 2, Truong San Phan 1 5, Jennifer Fleming ...
b, H&E staining of one young (upper panel) and one old (lower panel) liver specimen used for spatial transcriptomics (scale bar, 500 µm) and plots showing the expression levels of Glul, Cyp2f2 and Cyp2e1 indicated by color. The color gradient represents normalized gene expression. ...
First, a 1 ml syringe (Terumo, Tokyo, Japan) was cut half in length, and then the smaller hole located in the head of the syringe was covered with Parafilm®. Next, a 1.5 ml tube containing 1 × 106hiPSC-imSMC or hiPSC-mSMC cell pellet was prepared while 400 µl DM...
The bottom panel highlights the lesion interior. Scale bars, 100μm (top) and 50 μm (bottom). Metastatic lesions, blue dashed lines. l Quantification of NOS2+ staining detected by IHC and shown as percentage of individual control (n = 248), BG-treated (n = 173), BG +...
structure of def and relative position of targeting vector replacement in the def locus (second panel), generation of deffrt*loxp mice (third panel), generation of defloxp/loxp mice (four panel) and of conditional knockout of def in hepatocytes in defloxp/loxp,Alb-cre mice (bottom panel)....
Dissected endoderm gut tube segments were washed in cold DMEM/F12, then dissociated at 37°C for 20 min in accutase/0.25% trypsin (1:2). E9.5 and E10.5 single-cell suspensions were generated by dissection of liver buds, including STM, in PBS with fine dissecting forceps, ensuring removal ...
Is it putrefied? Is it both? I don’t know.Short of having a whole panel of fermentation experts intimately review the entire process, I’m not sure we’ll ever know. I seriously doubt GPP is willing to open up their entire process to that level of scrutiny. ...
2c, middle and right panel). Significant increase of these cells in male mice than in female was confirmed by quantitative analysis for F4/80- or CD68-positive cells (Fig. 2d). In the chow groups, these cells were rarely detected (Supplementary Fig. 3, middle and right panel). Therefore...
BM was flushed from the femur and tibia bones by cutting the tips of the bones and placing each bone in a 0.5-mL microfuge tube with a hole in the bottom using a 25-gauge needle. The bone was positioned with the trimmed bone tip directed to the hole in the tube, which was then pla...