List of species specific synonymous codon usage in F gene of Newcastle disease virus. N is the number of codons; RSCU is cumulative relative synonymous codon usage.Chandra, Shekhar KumarSachin, Kumar
The MCS in pECFP-C1 is between the ECFP coding sequence and the stop codon. Genes cloned into the MCS will be expressed as fusions to the C-terminus of ECFP if they are in the same reading frame as ECFP and there are no intervening in-frame stop codons. ECFP with a C-terminal fusion...
The MCS in pECFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the ECFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of ECFP if they are in the same reading frame as ECFP and there are no intervening stop codons. The ...
stop codons and an SV40 late polyadenylation region (Figures 4 and 5). The fusion gene region is flanked by T7 and T3 RNA polymerase promoters for the purpose of synthesizing sense and antisense RNA products, respectively. The Renilla luciferase gene on this vector is preceded by the SV40 ear...
/enhancer followed by a multiple cloning site (MCS) that precedes stop codons in all three reading frames, a synthetic intron known to enhance the stability of the mRNA, the ECMV IRES followed by the hygromycin B phosphotransferase gene, and the polyadenylation signal from SV40. Ribosomes can ...
the immediate early promoter of CMV (PCMV IE) and the AcGFP1 coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of AcGFP1 if they are in the same reading frame as AcGFP1 and there are no intervening stop codons. SV40 polyadenylation signals ...
the immediate early promoter of CMV (PCMV IE) and the DsRed-Monomer coding sequence. Genes cloned into the MCS are expressed as fusions to the N-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus ...
signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed1 if they are in the same reading frame as DsRed1 and there are no intervening stop codons. SV40 poly A signals downstream of the MCS direct proper processing of the 3' end of ...
sequences. Genes cloned into the MCS will be expressed as fusions to the N terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP...
into the MCS will be expressed as fusions to the C-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector ...