Biological materials represent a major source of inspiration to engineer protein-based polymers that can replicate the properties of living systems. Combined with our ability to control the molecular structure of proteins at the single amino acid level, this results in a vast array of attractive ...
Liquid–liquid phase separation of proteins underpins the formation of membraneless compartments in living cells. Elucidating the molecular driving forces underlying protein phase transitions is therefore a key objective for understanding biological function and malfunction. Here we show that cellular proteins,...
Liquid-liquid phase separation of proteins occurs on both surfaces of cellular membranes during diverse physiological processes. In vitro reconstitution could provide insight into the mechanisms underlying these events. However, most existing reconstitut
Prediction of Liquid-Liquid Phase Separation Proteins Using Machine LearningThe liquid-liquid phase separation (LLPS) of biomolecules in cell underpins the formation of membraneless organelles, which are the condensates of protein, nudoi:10.2139/ssrn.3515387Sun, Tanlin...
This makes the EM determination of individual species sometimes difficult, if not impossible. With the current study we demonstrate that liquid phase electrophoretic separation of proteins (as exemplary analytes) occurs in the capillary (capillary zone electrophoresis, CE) of the nano ES unit of the...
We show here that PrP undergoes LLPS, and that the PrP interaction with NAs modulates phase separation and promotes PrP fibrillation in a NA structure and concentrationヾependent manner. These results shed new light on the roles of NAs in PrP misfolding and TSEs....
1, 2, 3 Liquid-liquid phase separation (LLPS) of proteins underlies the formation of membraneless compartments in cells,4,5 possessing important intercellular functions,6, 7, 8 although aberrant LLPS and the liquid-solid phase separation (LSPS) can lead to formation of neurotoxic aggregates.9,...
Molecular Dynamics Simulations of Liquid-Liquid Phase Separation in Biology Cells are compartmentalized by numerous membrane-enclosed organelles and membraneless compartments to ensure that a wide variety of cellular activities occ... I Seim,A Gladfelter,D Klotsa - 《Bulletin of the American Physical ...
Compartmentalization of biochemical processes is essential for cell function. While membrane‐bound organelles are well﹕tudied in this context, recent work has shown that phase separation is a key contributor to cellular compartmentalization through the formation of liquid﹍ike membraneless organelles (...
A molecular grammar governing the driving forces for phase separation of prion-like RNA binding proteins. Cell 174, 688–699 (2018). CAS PubMed Central Google Scholar Murthy, A. C. et al. Molecular interactions underlying liquid–liquid phase separation of the FUS low-complexity domain. Nat....