包含一瓶 Lipofectamine™ 3000 试剂和一瓶 P3000TM 增强剂试剂。储存在 2–8°C 下。切勿冷冻。 Have questions about this product? Ask our AI assisted search. Why are my transfections not reproducible? Do I need to use the P3000 Reagent when transfecting siRNA? What if I am transfecting ...
Figure 2.The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. Download the Lipofectamine 3000 reagent protocol A gentle reagent with low transfection toxicity ...
TRANSFECTION PROTOCOL GCT Lung cancer cells Lipofectamine 3000 Transfection Reagent Complete growth medium Component Gibco™ RPMI 1640 Medium with GlutaMAX™ Supplement 10% Gibco™ FBS 25 mM Gibco™ HEPES (1 M) 1.0 mM Gibco™ Sodium Pyruvate Cat. No. 61870036 A3160401 15630080 11360070 ...
Protocol Figure 2. Enhanced and simple transfection protocol for a variety of cell lines. Seed cells to be 70–90% confluent at transfection Dilute Lipofectamine 3000 reagent in Opti-MEM medium—mix well Prepare master mix of DNA by diluting DNA in Opti-MEM medium, then add P3000 reagent—...
Lipofectamine® RNAiMAX Reagent Protocol 方案 RNAiMAX Forward Transfections Lipofectamine RNAiMAX Reverse Transfections Lipofectamine RNAiMAX Transfection for HUVEC Lipofectamine Transfecting Stealth using Lipofectamine® RNAiMAX 引用和文献(67) 搜索 引用和文献 ...
Lipofectamine 3000 Reagent Protocol (Chinese) 参考文献 本产品可用于的实验 公司简介 北京杰辉博高生物技术有限公司依托*重点实验室建立,是由具有资深生命科学、生物技术背景及留学归国人员共同创办的高新技术企业。 公司专业经营生物科学领域的实验仪器、研究试剂和实验室耗材,致力于向国内外生物科学研究人员提供 的服务...
Lipofectamine 3000 Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/lipofectamine 3000 transfection reagent/product/Thermo...
lipofectamine3000_scaling
Lipofectamine 3000 Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/lipofectamine 3000 transfection reagent/product/Thermo...
We use lipofectamine 3000 or 2000, but polyethylenimine (PEI) can also be used. See the following references for Lipofectamine and PEI https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/CellCultureandTransfection/pdfs/Lipofectamine_3000_Protocol_6Dec2013.pdf (Longo, Kavran, ...