c.1237-28A>G induces a deletion of exon 10, with no shift in reading frame leading to a protein of 858 amino acids. After expression in HeLa and HepG2 cells both MTP mutants localized in the ER despite not binding to protein disulfideisomerase. The two mutants induced a loss ofMTP TG...
According to this model, the virion assembly starts by binding of three specific sites of the circular dsDNA with one of the cap structures; this forms three loops, which gradually intertwine directed by protein assembly into the tubular structure; after all DNA has been covered by protein, ...
fold higher (Kaaround 3 × 104 M-1at 19 °C)47, justifying its strong binding to this LOS. Overall, the results clearly point to a lectin- and strain-selective recognition of theH. influenzaeLOSs, posing the question on the impact of this behaviour on the binding to the entire...
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Moreover, a CL spontaneously prolapsed out of the vagina 4 days after an emergency cesarean section for obstructed labor at 37 weeks; the woman had been conservatively treated during pregnancy. After a rubber ring pessary was placed, the tumor decreased in volume without symptoms after 6 weeks ...
Mllionl.eScpi.h2a01s9e, 2m0,oxleFOcuRlPaErEdRisRpEeVrIsEiWon in the lipid matrix after cooling [27]. Finally, the appeari4nogf 2o6f a well-shaped exothermic pick in the Lipomatrix sample around 220 ◦C, not recognized in none of the single components thermograms, could means that a...
TAGFILES = # When a file name is specified after GENERATE_TAGFILE, doxygen will create a # tag file that is based on the input files it reads. See section "Linking to # external documentation" for more information about the usage of tag files. GENERATE_TAGFILE = ...
(2) Methods: human liver cells (HepaRG), primary murine hepatocytes and Caco-2 intestinal epithelial cells were exposed to CdCl2before and after induction of lipotoxicity with oleic acid (OA) and/or palmitic acid (PA), and in some experiments, FA was combined with MLT (50 nM) treatment....
For MTT test, MTT (0.5 mg/mL) was added after the indicated treatment and incubated at 37 ˝C for 4 h. MTT test was performed to detect absorbance at 550 nm by microplate reader (M2, MD, CA). In Hoechst staining, cells were stained with Hoechst staining solution (beyotime, C1011)...
After 1 h incubation at 37 °C, the medium was removed, and the cells were washed three times with PBS. Cells were lysed in 300 µL of radioimmunoprecipitation assay (RIPA) buffer and incubation for 10 min at 37 °C. Radioactivity counts were measured in cell lysates using a Packard ...