using C11 BODIPY581/591. The treatment of cells with 0.1 µM RSL3 induced no increase in lipid peroxidation compared with the level found in the control group. However, more than 80% of cells cotreated with darapladib and RSL3 showed oxidised C11-BODIPY, as evidenced by flow cytometry...
In NEO cells, the cellular uptake of oxidized VLDL is ineffective because of the low LPL level and this seems to protect these cells from oxidized VLDL during a short exposure time used in these experiments (18 h) (Fig. 5). However, incubation of NEO cells with oxidized VLDL for longer ...
These results most likely reflect processing of HDL particles by elevated lipolytic activity (achieved by high transgene expression). The lipolytic processing, in turn, leads to dissociation of apoA-I and loss of protein from the circulation independently of selective uptake. In addition, our ...
The present study explored the high-level expression of RM lipase using superfolder green fluorescent protein (sfGFP)-mediated Escherichia coli secretion system. Results The sfGFP(−15) mutant was fused to the C-terminus of RM lipase to mediate its secretion expression. The yield of the fusion ...
Six-hour incubation with endothelial cells with ILO did not significantly decrease its level in conditioned medium (Fig. 2E). At the same time, 6-hr incubation with ILO-PC slightly decreased the levels of ILO-PC (less than 10%) in conditioned medium, but led to ILO-PC accumulation in ...
The preceding experiments suggest that the Gα subunits from the Gq family contribute to elevated PKD phosphorylation. To examine in more detail the stimulation of PKD by G protein signaling, we tested different Gq-, Gs- and Gi-coupled receptors for their ability to activate PKD1 in HEK 293 ...
In vivo experiments revealed that 29 slightly increased 2-AG levels, however, 30 dramatically elevated 2-AG levels. The discrepancy between those in vivo and in vitro experiments might be due to the stability of compounds 29 and 30. Rapid dilution studies have suggested that 30 might inhibit ...
Univariate analysis identified the tumor stage (<0.001), CA19-9 level (elevated or not, p = 0.009), the administration of chemotherapy (chemotherapy vs. no treatment, p = 0.006) and the preoperative LAR (<2.74, ≥2.74, p = 0.01) as poor prognostic factors regarding CSS in this study ...
For its potential use at an industrial level, the lipase production as a methanol tolerant whole-cell biocatalyst suitable for biodiesel production through transesterification reaction must be explored at a larger scale. Thus, the scale-up of lipase production byM. spiciferin a stirred-tank bioreacto...
Mildly elevated serum triglycerides are more common, with over one-third of Americans suffering from some level of hypertriglyceridemia (3). Hypertriglyceridemia is associated with increased risk of cardiovascular disease (4) along with predicting the development of type 2 diabetes mellitus (5). ...